Expression of transporter genes in astrocytes for different neuromediators in different brain departments in rats
Filyakova N. A., Patrusheva V. E., Patrushev M. V., Shusharina N. N., Silina E. V.
Journal of Pharmaceutical Sciences and Research
Vol.10, Issue5, P. 1297-1305
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
An experimental study has been carried out aiming at studying the expression of solute carriers (SLC) superfamily of the following neuromediators: glutamate, aspartate, lactate and choline. The study has been conducted on the pure culture of astroglial cells isolated from the brain in 3-day and 11-day rats. Expression of transporter genes in astrocytes for different neuromediators studied in different rat brain regions: cortex, brainstem, hippocampus. Results of the analysis of high-performance sequencing data are presented, which show that the expression of SLC transporters of neurometransmiters (glutamate, aspartate, lactate and choline) in different parts of the rat brain increases with the development of the organism, and this process occurs unevenly. An expression of glutamate and aspartate transporters in the brainstem of 3-day rats is higher than those in the other regions. An opposite effect is observed in rats at the age of eleven days. As they get older the expression of lact
Ключевые слова:
Aspartate; Astrocytes; Choline; Gene; Genetic methods; Glutamate; Lactate; Neuroglia; Neuromediators; Neurotransmitters; Transporters
aspartic acid; choline; excitatory amino acid transporter; glutamic acid; lactic acid; messenger RNA; neurotransmitter; organic cation transporter 1; organic cation transporter 2; organic cation transporter 3; solute carrier protein; agar gel electrophoresis; animal experiment; Article; astrocyte; brain cortex; brain ischemia; brain stem; cell suspension; degenerative disease; female; gene; gene expression; gene sequence; high throughput sequencing; hippocampus; immunomagnetic separation; male; nervous system development; neurologic disease; nonhuman; rat; RNA isolation; SLC transporter gene
Язык текста: Английский
ISSN: 0975-1459
Filyakova N. A.
Patrusheva V. E.
Patrushev M. V.
Shusharina N. N.
Silina E. V. Ekaterina Vladimirovna 1980-
Филякова Н. А.
Патрушева В. Е.
Патрушев М. В.
Шушарина Н. Н.
Силина Е. В. Екатерина Владимировна 1980-
Expression of transporter genes in astrocytes for different neuromediators in different brain departments in rats
Текст визуальный непосредственный
Journal of Pharmaceutical Sciences and Research
Vol.10, Issue5 P. 1297-1305
2018
Статья
Aspartate Astrocytes Choline Gene Genetic methods Glutamate Lactate Neuroglia Neuromediators Neurotransmitters Transporters
aspartic acid choline excitatory amino acid transporter glutamic acid lactic acid messenger RNA neurotransmitter organic cation transporter 1 organic cation transporter 2 organic cation transporter 3 solute carrier protein agar gel electrophoresis animal experiment Article astrocyte brain cortex brain ischemia brain stem cell suspension degenerative disease female gene gene expression gene sequence high throughput sequencing hippocampus immunomagnetic separation male nervous system development neurologic disease nonhuman rat RNA isolation SLC transporter gene
An experimental study has been carried out aiming at studying the expression of solute carriers (SLC) superfamily of the following neuromediators: glutamate, aspartate, lactate and choline. The study has been conducted on the pure culture of astroglial cells isolated from the brain in 3-day and 11-day rats. Expression of transporter genes in astrocytes for different neuromediators studied in different rat brain regions: cortex, brainstem, hippocampus. Results of the analysis of high-performance sequencing data are presented, which show that the expression of SLC transporters of neurometransmiters (glutamate, aspartate, lactate and choline) in different parts of the rat brain increases with the development of the organism, and this process occurs unevenly. An expression of glutamate and aspartate transporters in the brainstem of 3-day rats is higher than those in the other regions. An opposite effect is observed in rats at the age of eleven days. As they get older the expression of lact