Аннотация:
One of the essential goals in regenerative medicine is microvascularization which enables an effective blood supply within de novo constructed tissues and organs. In our study, we used two common multipotent mesenchymal stromal cell (MMSC) sources (subcutaneous adipose tissue and Wharton's jelly of the umbilical cord) where is a subpopulation of endothelial precursors. In the medium supplemented with VEGF, the 3D cultures of UC MMSCs and ADSCs promoted the endothelial cell differentiation. To evaluate their ability to form a capillary-like network, we encapsulated spheroids within non-modified and PEGylated fibrin hydrogels. The PEGylated hydrogel supported better the formation of multibranched cords than the pure fibrin gel. Analysis of tubule growth rate, length, and branching showed that the differentiated ADSCs had higher angiogenic potential than the differentiated hUC MMSCs. Our study can be a basis for the development of new strategies in tissue engineering and treatment of vasc
Gorkun A. A. Anastasiya Alekseevna 1986-
Shpichka A. I. Anastasiya Iosifovna 1991-
Zurina I. M. Irina Mikhaylovna 1988-
Koroleva A. V. Anastasiya Valeryevna 1984-
Kosheleva N. V.
Nikishin D. A.
Butnaru D. V. Denis Viktorovich 1978-
Timashev P. S. Petr Sergeevich 1978-
Repin V. S.
Saburina I. N.
Горкун А. А. Анастасия Алексеевна 1986-
Шпичка А. И. Анастасия Иосифовна 1991-
Зурина И. М. Ирина Михайловна 1988-
Королева А. В. Анастасия Валерьевна 1984-
Кошелева Н. В.
Никишин Д. А.
Бутнару Д. В. Денис Викторович 1978-
Тимашев П. С. Петр Сергеевич 1978-
Репин В. С.
Сабурина И. Н.
Angiogenic potential of spheroids from umbilical cord and adipose-derived multipotent mesenchymal stromal cells within fibrin gel
Текст визуальный непосредственный
Biomedical Materials (Bristol): Materials for tissue engineering and regenerative medicine
Institute of Physics and IOP Publishing Limited
Vol.13, Issue4 Num.044108
2018
Статья
angiogenesis fibrin mesenchymal stromal cells PEGylated fibrin spheroids tissue engineering vasculogenesis
Cytology Endothelial cells Hydrogels Synchrotrons Tissue Tissue engineering Uranium compounds Angiogenesis fibrin Mesenchymal stromal cells Pegylated spheroids Vasculogenesis Cell engineering beta1 integrin CD11b antigen CD14 antigen CD34 antigen complementary DNA endoglin fibrin genomic DNA platelet endothelial cell adhesion molecule 1 receptor type tyrosine protein phosphatase C Thy 1 membrane glycoprotein vasculotropin fibrin adipose derived stem cell angiogenesis Article cell differentiation cell migration DNA synthesis female flow cytometry gel growth rate human human cell hydrogel immunocytochemistry immunophenotyping mesenchymal stroma cell monolayer culture primary cell culture regenerative medicine reverse transcription polymerase chain reaction RNA isolation scanning electron microscopy subcutaneous fat time-lapse microscopy tumor spheroid umbilical cord vascularization Wharton jelly adipocyte cell culture technique cel
One of the essential goals in regenerative medicine is microvascularization which enables an effective blood supply within de novo constructed tissues and organs. In our study, we used two common multipotent mesenchymal stromal cell (MMSC) sources (subcutaneous adipose tissue and Wharton's jelly of the umbilical cord) where is a subpopulation of endothelial precursors. In the medium supplemented with VEGF, the 3D cultures of UC MMSCs and ADSCs promoted the endothelial cell differentiation. To evaluate their ability to form a capillary-like network, we encapsulated spheroids within non-modified and PEGylated fibrin hydrogels. The PEGylated hydrogel supported better the formation of multibranched cords than the pure fibrin gel. Analysis of tubule growth rate, length, and branching showed that the differentiated ADSCs had higher angiogenic potential than the differentiated hUC MMSCs. Our study can be a basis for the development of new strategies in tissue engineering and treatment of vasc