Effect of Curcumin and Gliotoxin on Rat Liver Myofibroblast Culture
Shafigullina A. K., Miyanovich O., Prottoy R. A., Zhuravleva M. N., Gomzikova M. O., Gumerova A. A., Rizvanov A. A., Kiyasov A. P.
BioNanoScience
Vol.8, Issue2, P. 522-536
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1007/s12668-017-0494-z
Аннотация:
Since the 1990s, when it was demonstrated by Hammel and others that liver fibrosis is reversible, researchers and physicians actively search for new antifibrotic therapies. In recent years, knowledge of liver fibrosis pathophysiology has greatly advanced and new cellular and molecular mechanisms were described. The cells that determine extracellular matrix components distribution are myofibroblasts, but their origin is diverse. They can be activated hepatic stellate cells (HSCs), portal fibroblasts (PF), or circulating mesenchymal stem cells of the bone marrow. Among large number of substrates to inhibit activation, to inhibit proliferation of myofibroblasts, and to induce their apoptosis we, chose curcumin and gliotoxin. Primarily, in the current work, we optimized the explantation culture method for isolation of hepatic myofibroblasts and received two different cultures—myofibroblasts of HSC and PF origin. Exposition of 50 μM curcumin and 0.1 μM gliotoxin was the most optimal; we obs
Ключевые слова:
Apoptosis; Curcumin; Gliotoxin; Hepatic stellate cells; Liver fibrosis; Myofibroblasts; Portal fibroblasts
Cell culture; Cell death; Chemical activation; Stem cells; Curcumin; Gliotoxin; Hepatic stellate cells; Liver fibrosis; Myofibroblasts; Portal fibroblasts; Fibroblasts; curcumin; gliotoxin; animal cell; animal experiment; animal model; apoptosis; Article; bone marrow; cell proliferation; extracellular matrix; flow cytometry; hepatic stellate cell; immunoblotting; immunocytochemistry; liver fibrosis; liver parenchyma; mesenchymal stem cell; myofibroblast; nonhuman; rat; Western blotting
Язык текста: Английский
ISSN: 2191-1649
Shafigullina A. K.
Miyanovich O. Olya 1978-
Prottoy R. A.
Zhuravleva M. N.
Gomzikova M. O.
Gumerova A. A.
Rizvanov A. A.
Kiyasov A. P.
Шафигуллина А. К.
Миянович О. Оля 1978-
Проттоy Р. А.
Журавлева М. Н.
Гомзикова М. О.
Гумерова А. А.
Ризванов А. А.
Киясов А. П.
Effect of Curcumin and Gliotoxin on Rat Liver Myofibroblast Culture
Текст визуальный непосредственный
BioNanoScience
Vol.8, Issue2 P. 522-536
2018
Статья
Apoptosis Curcumin Gliotoxin Hepatic stellate cells Liver fibrosis Myofibroblasts Portal fibroblasts
Cell culture Cell death Chemical activation Stem cells Curcumin Gliotoxin Hepatic stellate cells Liver fibrosis Myofibroblasts Portal fibroblasts Fibroblasts curcumin gliotoxin animal cell animal experiment animal model apoptosis Article bone marrow cell proliferation extracellular matrix flow cytometry hepatic stellate cell immunoblotting immunocytochemistry liver fibrosis liver parenchyma mesenchymal stem cell myofibroblast nonhuman rat Western blotting
Since the 1990s, when it was demonstrated by Hammel and others that liver fibrosis is reversible, researchers and physicians actively search for new antifibrotic therapies. In recent years, knowledge of liver fibrosis pathophysiology has greatly advanced and new cellular and molecular mechanisms were described. The cells that determine extracellular matrix components distribution are myofibroblasts, but their origin is diverse. They can be activated hepatic stellate cells (HSCs), portal fibroblasts (PF), or circulating mesenchymal stem cells of the bone marrow. Among large number of substrates to inhibit activation, to inhibit proliferation of myofibroblasts, and to induce their apoptosis we, chose curcumin and gliotoxin. Primarily, in the current work, we optimized the explantation culture method for isolation of hepatic myofibroblasts and received two different cultures—myofibroblasts of HSC and PF origin. Exposition of 50 μM curcumin and 0.1 μM gliotoxin was the most optimal; we obs