Investigation of ranges and frequency of mutations in the embB gene in mycobacterium tuberculosis associated with resistance to ethambutol using...
Аляпкина Ю. С., Larionova E. E., Смирнова Т. Г., Алексеев Y. А. И., Черноусова Л. Н., Vladimirskiy M. A.
Туберкулез и болезни легких
Т. 95, Вып. 11, С. 27-35
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.21292/2075-1230-2017-95-11-27-35
Аннотация:
Based on real-time allele-specific polymerase chain reaction, the ranges of potential mutations in codons of 306 and 405 of the embB gene in Mycobacterium tuberculosis associated with resistance to ethambutol were investigated 5 different mutations were detected in codon 306 and 3 mutations were found in codon 406 of the embB gene The detected mutations were confirmed by sequencing and mass spectrometry By analyzing the frequency of detected mutations of, the set of reagents was developed for rapid testing of susceptibility tuberculous mycobacteria to ethambutol by multi-competitive allele-specific real-time PCR Out of 107 tested specimens of clinical isolates, mutations of the embB gene of M. tuberculosis were detected in 49 (458[%]) specimens, and no mutations were found in 58 (522[%]) specimens 39 (364[%]) specimens had mutations in codon 306 of the embB gene, and 9 (84[%]) specimens had a mutation in codon 406, and 1 (09[%]) specimen had mutations in both codons 306 and 406 The high level of
Ключевые слова:
Drug resistance; Ethambutol; Real-time allele-specific PCR; Tuberculous mycobacteria
Язык текста: Русский
ISSN: 2542-1506
Аляпкина Ю. С. Юлия Сергеевна 1967-
Larionova E. E.
Смирнова Т. Г.
Алексеев Y. А. И.
Черноусова Л. Н.
Vladimirskiy M. A.
Alyapkina Yu. S. Yuliya Sergeevna 1967-
Ларионова Е. Е.
Smirnova T. G.
Alekseev Y. A. I.
Chernousova L. N.
Владимирскиy М. А.
Investigation of ranges and frequency of mutations in the embB gene in mycobacterium tuberculosis associated with resistance to ethambutol using real-time polymerase chain reaction
Investigation of ranges and frequency of mutations in the embB gene in mycobacterium tuberculosis associated with resistance to ethambutol using...
Текст визуальный непосредственный
Туберкулез и болезни легких
Нью Терра
Т. 95, Вып. 11 С. 27-35
2017
Статья
Drug resistance Ethambutol Real-time allele-specific PCR Tuberculous mycobacteria
Based on real-time allele-specific polymerase chain reaction, the ranges of potential mutations in codons of 306 and 405 of the embB gene in Mycobacterium tuberculosis associated with resistance to ethambutol were investigated 5 different mutations were detected in codon 306 and 3 mutations were found in codon 406 of the embB gene The detected mutations were confirmed by sequencing and mass spectrometry By analyzing the frequency of detected mutations of, the set of reagents was developed for rapid testing of susceptibility tuberculous mycobacteria to ethambutol by multi-competitive allele-specific real-time PCR Out of 107 tested specimens of clinical isolates, mutations of the embB gene of M. tuberculosis were detected in 49 (458[%]) specimens, and no mutations were found in 58 (522[%]) specimens 39 (364[%]) specimens had mutations in codon 306 of the embB gene, and 9 (84[%]) specimens had a mutation in codon 406, and 1 (09[%]) specimen had mutations in both codons 306 and 406 The high level of