A novel marsupial hepatitis A virus corroborates complex evolutionary patterns shaping the genus Hepatovirus
Carneiro I. O., Sander A. -., Silva N., Moreira-Soto A., Normann A., Flehmig B., Lukashev A. N., Dotzauer A., Wieseke N., Franke C. R., Doktor D. Ya. F.
Journal of Virology
Vol.92, Issue13, Num.e00082-18
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
The discovery of highly diverse nonprimate hepatoviruses illuminated the evolutionary origins of hepatitis A virus (HAV) ancestors in mammals other than primates. Marsupials are ancient mammals that diverged from other Eutheria during the Jurassic. Viruses from marsupials may thus provide important insight into virus evolution. To investigate Hepatovirus macroevolutionary patterns, we sampled 112 opossums in northeastern Brazil. A novel marsupial HAV (MHAV) in the Brazilian common opossum (Didelphis aurita) was detected by nested reverse transcription- PCR (RT-PCR). MHAV concentration in the liver was high, at 2.5 × 10 9 RNA copies/g, and at least 300-fold higher than those in other solid organs, suggesting hepatotropism. Hepatovirus seroprevalence in D. aurita was 26.6[%] as determined using an enzyme-linked immunosorbent assay (ELISA). Endpoint titers in confirmatory immunofluorescence assays were high, and marsupial antibodies colocalized with anti- HAV control sera, suggesting specif
Ключевые слова:
Evolution; Hepatitis A virus; Pathogenesis; Small mammals
antibody; CpG oligodeoxynucleotide; protein VP1; virus antibody; virus RNA; viral protein; virus antibody; Article; Brazil; carboxy terminal sequence; controlled study; enzyme linked immunosorbent assay; evolution; gene sequence; gene structure; Hepatitis A virus; Hepatovirus; immunofluorescence test; internal ribosome entry site; liver; Marsupial Hepatitis A virus; nonhuman; nucleotide sequence; opossum; phylogeny; placental mammal; plesiomorphy; priority journal; protein cleavage; protein processing; reverse transcription polymerase chain reaction; rodent; serology; seroprevalence; virus cell interaction; virus detection; virus titration; animal; chemistry; classification; genetics; Hepatitis A virus; immunology; marsupial; metabolism; molecular evolution; physiology; viral tropism; virology; Animals; Antibodies, Viral; Brazil; Evolution, Molecular; Hepatitis A virus; Liver; Marsupialia; Phylogeny; Viral Proteins; Viral Tropism
Язык текста: Английский
ISSN: 1098-5514
Carneiro I. O.
Sander A. -. A.-L.
Silva N.
Moreira-Soto A.
Normann A.
Flehmig B.
Lukashev A. N. Aleksandr Nikolaevich 1977-
Dotzauer A.
Wieseke N.
Franke C. R.
Doktor D. Ya. F. Dreksler Yan Feliks 1976-
Cарнеиро И. О.
Сандер А. -. А.-Л.
Силва Н.
Мореира-Сото А.
Норманн А.
Флехмиг Б.
Лукашев А. Н. Александр Николаевич 1977-
Дотзауер А.
Wиесеке Н.
Франке C. Р.
Доктор Д. Я. Ф. Дрекслер Ян Феликс 1976-
A novel marsupial hepatitis A virus corroborates complex evolutionary patterns shaping the genus Hepatovirus
Текст визуальный непосредственный
Journal of Virology
American Society for Microbiology
Vol.92, Issue13 Num.e00082-18
2018
Статья
Evolution Hepatitis A virus Pathogenesis Small mammals
antibody CpG oligodeoxynucleotide protein VP1 virus antibody virus RNA viral protein virus antibody Article Brazil carboxy terminal sequence controlled study enzyme linked immunosorbent assay evolution gene sequence gene structure Hepatitis A virus Hepatovirus immunofluorescence test internal ribosome entry site liver Marsupial Hepatitis A virus nonhuman nucleotide sequence opossum phylogeny placental mammal plesiomorphy priority journal protein cleavage protein processing reverse transcription polymerase chain reaction rodent serology seroprevalence virus cell interaction virus detection virus titration animal chemistry classification genetics Hepatitis A virus immunology marsupial metabolism molecular evolution physiology viral tropism virology Animals Antibodies, Viral Brazil Evolution, Molecular Hepatitis A virus Liver Marsupialia Phylogeny Viral Proteins Viral Tropism
The discovery of highly diverse nonprimate hepatoviruses illuminated the evolutionary origins of hepatitis A virus (HAV) ancestors in mammals other than primates. Marsupials are ancient mammals that diverged from other Eutheria during the Jurassic. Viruses from marsupials may thus provide important insight into virus evolution. To investigate Hepatovirus macroevolutionary patterns, we sampled 112 opossums in northeastern Brazil. A novel marsupial HAV (MHAV) in the Brazilian common opossum (Didelphis aurita) was detected by nested reverse transcription- PCR (RT-PCR). MHAV concentration in the liver was high, at 2.5 × 10 9 RNA copies/g, and at least 300-fold higher than those in other solid organs, suggesting hepatotropism. Hepatovirus seroprevalence in D. aurita was 26.6[%] as determined using an enzyme-linked immunosorbent assay (ELISA). Endpoint titers in confirmatory immunofluorescence assays were high, and marsupial antibodies colocalized with anti- HAV control sera, suggesting specif