A new sample treatment for asialo-Tf determination with capillary electrophoresis: an added value to the analysis of CDT
Porpiglia N. M., De P. E. F., Savchuk S. A., Appolonova S. A., Bortolotti F., Tal`yaro F.
Clinica Chimica Acta
Vol.483, P. 256-262
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1016/j.cca.2018.05.019
Аннотация:
Background and aim: The non-glycosylated glycoform of transferrin (Tf), known as asialo-Tf, was not selected (in favor of disialo-Tf) as the measurand for the standardization of carbohydrate deficient transferrin (CDT) determination because of a lower diagnostic sensitivity provided with the currently available analytical procedures for sera. However, asialo-Tf could provide an additional value to disialo-Tf in the CDT analysis employed in forensic toxicology contexts. The present work aimed at developing an easy sample preparation based on PEG precipitation in order to improve the detectability of asialo-Tf in capillary electrophoresis (CE). Methods: Equal volumes (35 μL) of serum and of 30[%] PEG-8000 were mixed and briefly vortexed. After centrifugation, the supernatant was iron saturated with a ferric solution (1:1, v/v). The mixture was analyzed in CE for asialo-Tf and disialo-Tf determination. Results: PEG-8000 precipitation allowed the improvement of the baseline in the electrophe
Ключевые слова:
Alcohol abuse; Asialo-Tf; Capillary electrophoresis; CDT; Forensic toxicology; PEG
alcohol; asialo transferrin; biological marker; carbohydrate deficient transferrin; disialo Tf; iron; macrogol 8000; transferrin; unclassified drug; carbohydrate-deficient transferrin; macrogol; macrogol 8000; transferrin; Article; capillary electrophoresis; centrifugation; controlled study; forensic toxicology; human; intermethod comparison; precipitation; priority journal; protein blood level; protein determination; serum; supernatant; alcoholism; analogs and derivatives; blood; capillary electrophoresis; procedures; specimen handling; Alcoholism; Biomarkers; Chemical Precipitation; Electrophoresis, Capillary; Forensic Toxicology; Humans; Polyethylene Glycols; Specimen Handling; Transferrin
Язык текста: Английский
ISSN: 1873-3492
Porpiglia N. M.
De P. E. F. Palo E.F.
Savchuk S. A. Sergej Aleksandrovich 1958-
Appolonova S. A. Svetlana Aleksandrovna 1973-
Bortolotti F.
Tal`yaro F. Franko 1952-
Порпиглиа Н. М.
Де П. Е. Ф. Пало Е.Ф.
Савчук С. А. Сергей Александрович 1958-
Апполонова С. А. Светлана Александровна 1973-
Бортолотти Ф.
Тальяро Ф. Франко 1952-
A new sample treatment for asialo-Tf determination with capillary electrophoresis: an added value to the analysis of CDT
Текст визуальный непосредственный
Clinica Chimica Acta
Elsevier Science Publisher B.V.
Vol.483 P. 256-262
2018
Статья
Alcohol abuse Asialo-Tf Capillary electrophoresis CDT Forensic toxicology PEG
alcohol asialo transferrin biological marker carbohydrate deficient transferrin disialo Tf iron macrogol 8000 transferrin unclassified drug carbohydrate-deficient transferrin macrogol macrogol 8000 transferrin Article capillary electrophoresis centrifugation controlled study forensic toxicology human intermethod comparison precipitation priority journal protein blood level protein determination serum supernatant alcoholism analogs and derivatives blood capillary electrophoresis procedures specimen handling Alcoholism Biomarkers Chemical Precipitation Electrophoresis, Capillary Forensic Toxicology Humans Polyethylene Glycols Specimen Handling Transferrin
Background and aim: The non-glycosylated glycoform of transferrin (Tf), known as asialo-Tf, was not selected (in favor of disialo-Tf) as the measurand for the standardization of carbohydrate deficient transferrin (CDT) determination because of a lower diagnostic sensitivity provided with the currently available analytical procedures for sera. However, asialo-Tf could provide an additional value to disialo-Tf in the CDT analysis employed in forensic toxicology contexts. The present work aimed at developing an easy sample preparation based on PEG precipitation in order to improve the detectability of asialo-Tf in capillary electrophoresis (CE). Methods: Equal volumes (35 μL) of serum and of 30[%] PEG-8000 were mixed and briefly vortexed. After centrifugation, the supernatant was iron saturated with a ferric solution (1:1, v/v). The mixture was analyzed in CE for asialo-Tf and disialo-Tf determination. Results: PEG-8000 precipitation allowed the improvement of the baseline in the electrophe