Comparative effects of calcium and potassium channel modulators on ischemia/reperfusion injury in the isolated rat heart
Simonovic N., Yakovlevich V., Jeremic J., Finderle Z., Srejovic I., Nikolic T. T., Milosavljevic I., Zivkovic V.
Molecular and Cellular Biochemistry
Vol.450, Issue1-2, P. 175-185
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s11010-018-3384-y
Аннотация:
The aim of this study was to examine and compare the effects of the acute administration of verapamil or amlodipine as representatives of the calcium channel blockers or nicorandil as a representative of the mitochondrial ATP-dependent potassium (K ATP ) channel opener to cardiac contractility, coronary flow, and oxidative stress markers on ischemia/reperfusion injury in the isolated rat heart. The hearts of adult male Wistar albino rats (n = 60 total, 12 per group) were divided into five groups, two controls (preconditioning with Krebs–Henseleit solution) and three experimental depending on acute administrated pharmacological agents (0,63 µmol/L of verapamil, 0,1 µmol/L of amlodipine, and 200 µmol/L of nicorandil). After stabilization and 5 min of preconditioning in experimental groups, hearts from I/R control and all experimental groups underwent global ischemia (20 min) and reperfusion (30 min). Hearts from sham group were continuously followed for 50 min, after stabilization period
Ключевые слова:
Amlodipine; Ischemia/reperfusion; Nicorandil; Pharmacological preconditioning; Verapamil
adenosine triphosphatase (potassium); amlodipine; calcium channel; hydrogen peroxide; nicorandil; nitrite; potassium channel; radical; superoxide; thiobarbituric acid reactive substance; verapamil; adenosine triphosphatase (potassium sodium); calcium channel; calcium channel blocking agent; nicorandil; acute drug administration; adult; animal experiment; animal model; Article; comparative study; controlled study; coronary artery blood flow; diastolic blood pressure; drug effect; heart function; heart left ventricle; heart left ventricle pressure; heart muscle contractility; heart protection; heart rate; hemodynamics; ischemic preconditioning; isolated heart; lipid peroxidation; male; myocardial ischemia reperfusion injury; nonhuman; oxidative stress; rat; systolic blood pressure; Wistar rat; animal; antagonists and inhibitors; cardiac muscle; drug effect; heart contraction; metabolism; myocardial ischemia reperfusion injury; organ culture technique; pathology; Animals; Calcium Channel
Язык текста: Английский
ISSN: 1573-4919
Simonovic N.
Yakovlevich V. Vladimir 1971-
Jeremic J.
Finderle Z.
Srejovic I.
Nikolic T. T. Turnic T.
Milosavljevic I.
Zivkovic V.
Симоновиc Н.
Яковлевич В. Владимир 1971-
Йеремиc Й.
Финдерле З.
Срейовиc И.
Николиc Т. Т. Тюрниc Т.
Милосавлйевиc И.
Зивковиc В.
Comparative effects of calcium and potassium channel modulators on ischemia/reperfusion injury in the isolated rat heart
Текст визуальный непосредственный
Molecular and Cellular Biochemistry
Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Vol.450, Issue1-2 P. 175-185
2019
Статья
Amlodipine Ischemia/reperfusion Nicorandil Pharmacological preconditioning Verapamil
adenosine triphosphatase (potassium) amlodipine calcium channel hydrogen peroxide nicorandil nitrite potassium channel radical superoxide thiobarbituric acid reactive substance verapamil adenosine triphosphatase (potassium sodium) calcium channel calcium channel blocking agent nicorandil acute drug administration adult animal experiment animal model Article comparative study controlled study coronary artery blood flow diastolic blood pressure drug effect heart function heart left ventricle heart left ventricle pressure heart muscle contractility heart protection heart rate hemodynamics ischemic preconditioning isolated heart lipid peroxidation male myocardial ischemia reperfusion injury nonhuman oxidative stress rat systolic blood pressure Wistar rat animal antagonists and inhibitors cardiac muscle drug effect heart contraction metabolism myocardial ischemia reperfusion injury organ culture technique pathology Animals Calcium Channel
The aim of this study was to examine and compare the effects of the acute administration of verapamil or amlodipine as representatives of the calcium channel blockers or nicorandil as a representative of the mitochondrial ATP-dependent potassium (K ATP ) channel opener to cardiac contractility, coronary flow, and oxidative stress markers on ischemia/reperfusion injury in the isolated rat heart. The hearts of adult male Wistar albino rats (n = 60 total, 12 per group) were divided into five groups, two controls (preconditioning with Krebs–Henseleit solution) and three experimental depending on acute administrated pharmacological agents (0,63 µmol/L of verapamil, 0,1 µmol/L of amlodipine, and 200 µmol/L of nicorandil). After stabilization and 5 min of preconditioning in experimental groups, hearts from I/R control and all experimental groups underwent global ischemia (20 min) and reperfusion (30 min). Hearts from sham group were continuously followed for 50 min, after stabilization period