Proliferative Activity of Mouse Splenocytes in Physiological Pregnancy and in Models of Spontaneous and Muramylpeptide-Dependent Abortions
Artem’eva K. A., Boltovskaya M. N., Bogdanova I. M., Obernikhin S. S., Stepanova I. I., Stepanov A. A., Svitich O. A., Kalyuzhin O. V.
Bulletin of Experimental Biology and Medicine
Vol.168, Issue6, P. 757-760
Опубликовано: 2020
Тип ресурса: Статья
DOI:10.1007/s10517-020-04796-8
Аннотация:
Spontaneous proliferative activity of splenocytes in female CBA mice and the response of these cells to antigens of allogeneic male BALB/c and DBA/2 mice in a mixed splenocyte culture were evaluated by 3H-thymidine incorporation in different pregnancy models. ♀CBA×♂BALB/c mating was used for modeling physiological pregnancy. Spontaneous abortions were reproduced by abortion-prone ♀CBA×♂DBA/2 mating. In order to simulate immunostimulant-induced and immunostimulant-potentiated abortions, 0.83 mg/kg muramyl dipeptide β-heptylglycoside was intraperitoneally injected to CBA females mated with BALB/c or DBA/2 males, respectively, on gestation days 5 and 7. The increase in the rate of embryo resorption in the models of spontaneous, induced, and potentiated abortions occurred against the background of an increase in the level of spontaneous proliferation of splenocytes and a decrease in their reactivity to paternal antigens on gestation day 9. © 2020, Springer Science+Business Media, LLC, part
Ключевые слова:
induced abortions; mouse models of miscarriage; muramyl dipeptide β-heptylglycoside; proliferation of splenocytes; spontaneous abortions
antigen; muramyl peptide; animal cell; animal experiment; animal model; animal tissue; Article; Bagg albino mouse; cell proliferation; controlled study; DBA 2 mouse; DNA synthesis; embryo; embryo resorption; female; fetus; gestational age; male; mouse; mouse model; nonhuman; physiological process; pregnancy; spleen cell; spontaneous abortion
Язык текста: Английский
ISSN: 1573-8221
Artem’eva K. A.
Boltovskaya M. N.
Bogdanova I. M.
Obernikhin S. S. Sergej Stanislavovich 1962-
Stepanova I. I.
Stepanov A. A.
Svitich O. A. Oksana Anatolyevna 1979-
Kalyuzhin O. V. Oleg Vitalyevich 1970-
Артем’ева К. А.
Болтовскайа М. Н.
Богданова И. М.
Обернихин С. С. Сергей Станиславович 1962-
Степанова И. И.
Степанов А. А.
Свитич О. А. Оксана Анатольевна 1979-
Калюжин О. В. Олег Витальевич 1970-
Proliferative Activity of Mouse Splenocytes in Physiological Pregnancy and in Models of Spontaneous and Muramylpeptide-Dependent Abortions
Текст визуальный непосредственный
Bulletin of Experimental Biology and Medicine
Springer New York Consultants Bureau
Vol.168, Issue6 P. 757-760
2020
Статья
induced abortions mouse models of miscarriage muramyl dipeptide β-heptylglycoside proliferation of splenocytes spontaneous abortions
antigen muramyl peptide animal cell animal experiment animal model animal tissue Article Bagg albino mouse cell proliferation controlled study DBA 2 mouse DNA synthesis embryo embryo resorption female fetus gestational age male mouse mouse model nonhuman physiological process pregnancy spleen cell spontaneous abortion
Spontaneous proliferative activity of splenocytes in female CBA mice and the response of these cells to antigens of allogeneic male BALB/c and DBA/2 mice in a mixed splenocyte culture were evaluated by 3H-thymidine incorporation in different pregnancy models. ♀CBA×♂BALB/c mating was used for modeling physiological pregnancy. Spontaneous abortions were reproduced by abortion-prone ♀CBA×♂DBA/2 mating. In order to simulate immunostimulant-induced and immunostimulant-potentiated abortions, 0.83 mg/kg muramyl dipeptide β-heptylglycoside was intraperitoneally injected to CBA females mated with BALB/c or DBA/2 males, respectively, on gestation days 5 and 7. The increase in the rate of embryo resorption in the models of spontaneous, induced, and potentiated abortions occurred against the background of an increase in the level of spontaneous proliferation of splenocytes and a decrease in their reactivity to paternal antigens on gestation day 9. © 2020, Springer Science+Business Media, LLC, part