E2F6-mediated downregulation of MiR22HG facilitates the progression of laryngocarcinoma by targeting the miR-5000-3p/FBXW7 axis
Chen H., Ali M., Ruben A., Stelmakh D., Pak M.
Molecular and Cellular Biology
Vol.40, Issue10, Num.00496-19
Опубликовано: 2020
Тип ресурса: Статья
Аннотация:
Recently, abundant evidence has clarified that long noncoding RNAs (lncRNAs) play an oncogenic or anticancer role in the tumorigenesis and development of diverse human cancers. Described as a crucial regulator in some cancers, MIR22HG has not yet been studied in laryngocarcinoma and therefore the underlying regulatory role of MIR22HG in laryngocarcinoma is worth detecting. In this study, MIR22HG expression in laryngocarcinoma cells was confirmed to be downregulated, and upregulated MIR22HG expression led to suppressive effects on laryngocarcinoma cell proliferation and migration. Molecular mechanism assays revealed that MIR22HG sponges miR-5000-3p in laryngocarcinoma cells. Besides, decreased expression of miR-5000-3p suppressed laryngocarcinoma cell proliferation and migration. Moreover, the FBXW7 gene was reported to be a downstream target gene of miR-5000-3p in laryngocarcinoma cells. More importantly, rescue assays verified that FBXW7 depletion or miR-5000-3p upregulation counterva
Ключевые слова:
E2F6; FBXW7; Laryngocarcinoma; MiR-5000-3p; MIR22HG
biological marker; complementary DNA; F box/WD repeat containing protein 7; long untranslated RNA; microRNA; microRNA 5000 3p; transcription factor E2F6; unclassified drug; E2F6 protein, human; F box/WD repeat containing protein 7; FBXW7 protein, human; microRNA; MIRN22 microRNA, human; MIRN5000 microRNA, human; transcription factor E2F6; Article; cancer cell; cancer inhibition; carcinogenesis; cell migration assay; cell proliferation assay; gene expression; human; human cell; larynx carcinoma; priority journal; reverse transcription polymerase chain reaction; upregulation; Western blotting; cell proliferation; disease exacerbation; down regulation; gene expression regulation; genetics; larynx tumor; pathology; tumor cell line; tumor invasion; Cell Line, Tumor; Cell Proliferation; Disease Progression; Down-Regulation; E2F6 Transcription Factor; F-Box-WD Repeat-Containing Protein 7; Gene Expression Regulation, Neoplastic; Humans; Laryngeal Neoplasms; MicroRNAs; Neoplasm Invasiveness
Язык текста: Английский
ISSN: 1098-5549
Chen H.
Ali M.
Ruben A.
Stelmakh D.
Pak M.
Чен Х.
Али М.
Рубен А.
Стелмах Д.
Пак М.
E2F6-mediated downregulation of MiR22HG facilitates the progression of laryngocarcinoma by targeting the miR-5000-3p/FBXW7 axis
Текст визуальный непосредственный
Molecular and Cellular Biology
American Society for Microbiology
Vol.40, Issue10 Num.00496-19
2020
Статья
E2F6 FBXW7 Laryngocarcinoma MiR-5000-3p MIR22HG
biological marker complementary DNA F box/WD repeat containing protein 7 long untranslated RNA microRNA microRNA 5000 3p transcription factor E2F6 unclassified drug E2F6 protein, human F box/WD repeat containing protein 7 FBXW7 protein, human microRNA MIRN22 microRNA, human MIRN5000 microRNA, human transcription factor E2F6 Article cancer cell cancer inhibition carcinogenesis cell migration assay cell proliferation assay gene expression human human cell larynx carcinoma priority journal reverse transcription polymerase chain reaction upregulation Western blotting cell proliferation disease exacerbation down regulation gene expression regulation genetics larynx tumor pathology tumor cell line tumor invasion Cell Line, Tumor Cell Proliferation Disease Progression Down-Regulation E2F6 Transcription Factor F-Box-WD Repeat-Containing Protein 7 Gene Expression Regulation, Neoplastic Humans Laryngeal Neoplasms MicroRNAs Neoplasm Invasiveness
Recently, abundant evidence has clarified that long noncoding RNAs (lncRNAs) play an oncogenic or anticancer role in the tumorigenesis and development of diverse human cancers. Described as a crucial regulator in some cancers, MIR22HG has not yet been studied in laryngocarcinoma and therefore the underlying regulatory role of MIR22HG in laryngocarcinoma is worth detecting. In this study, MIR22HG expression in laryngocarcinoma cells was confirmed to be downregulated, and upregulated MIR22HG expression led to suppressive effects on laryngocarcinoma cell proliferation and migration. Molecular mechanism assays revealed that MIR22HG sponges miR-5000-3p in laryngocarcinoma cells. Besides, decreased expression of miR-5000-3p suppressed laryngocarcinoma cell proliferation and migration. Moreover, the FBXW7 gene was reported to be a downstream target gene of miR-5000-3p in laryngocarcinoma cells. More importantly, rescue assays verified that FBXW7 depletion or miR-5000-3p upregulation counterva