Аннотация:
Significance: Currently, various scaffolds with immobilized cells are widely used in tissue engineering and regenerative medicine. However, the physiological activity and cell viability in such constructs might be impaired due to a lack of oxygen and nutrients. Photobiomodulation (PBM) is a promising method of preconditioning cells to increase their metabolic activity and to activate proliferation or differentiation. Aim: Investigation of the potential of PBM for stimulation of cell activities in hydrogels. Approach: Mesenchymal stromal cells (MSCs) isolated from human gingival mucosa were encapsulated in modified fibrin hydrogels with different thicknesses and concentrations. Constructs with cells were subjected to a single-time exposure to red (630 nm) and near-infrared (IR) (840 nm) low-intensity irradiation. After 3 days of cultivation, the viability and physiological activity of the cells were analyzed using confocal microscopy and a set of classical tests for cytotoxicity. Result
Ключевые слова:
Fibrin; Hydrogel; Mesenchymal stromal cells; Photobiomodulation; Regenerative medicine; Tissue engineering
Cell culture; Cell engineering; Cells; Flowcharting; Hydrogels; Infrared devices; Irradiation; Metabolism; Physiology; Tissue; Cell viability; Classical tests; Immobilized cells; Low-intensity irradiation; Mesenchymal stromal cells; Metabolic activity; Photobiomodulation; Physiological activity; Scaffolds (biology)
Bikmulina P. Yu. Polina Yuryevna 1997-
Kosheleva N. V.
Shpichka A. I. Anastasiya Iosifovna 1991-
Efremov Yu. M. Yurij Mikhaylovich 1989-
Yusupov V. I.
Timashev P. S. Petr Sergeevich 1978-
Rochev Yu. A. Yurij Alekseevich 1961-
Бикмулина П. Ю. Полина Юрьевна 1997-
Кошелева Н. В.
Шпичка А. И. Анастасия Иосифовна 1991-
Ефремов Ю. М. Юрий Михайлович 1989-
Юсупов В. И.
Тимашев П. С. Петр Сергеевич 1978-
Рочев Ю. А. Юрий Алексеевич 1961-
Beyond 2D: Effects of photobiomodulation in 3D tissue-like systems
Текст визуальный непосредственный
Journal of Biomedical Optics
S P I E - International Society for Optical Engineering
Vol.25, Issue4 Num.048001
2020
Статья
Fibrin Hydrogel Mesenchymal stromal cells Photobiomodulation Regenerative medicine Tissue engineering
Cell culture Cell engineering Cells Flowcharting Hydrogels Infrared devices Irradiation Metabolism Physiology Tissue Cell viability Classical tests Immobilized cells Low-intensity irradiation Mesenchymal stromal cells Metabolic activity Photobiomodulation Physiological activity Scaffolds (biology)
Significance: Currently, various scaffolds with immobilized cells are widely used in tissue engineering and regenerative medicine. However, the physiological activity and cell viability in such constructs might be impaired due to a lack of oxygen and nutrients. Photobiomodulation (PBM) is a promising method of preconditioning cells to increase their metabolic activity and to activate proliferation or differentiation. Aim: Investigation of the potential of PBM for stimulation of cell activities in hydrogels. Approach: Mesenchymal stromal cells (MSCs) isolated from human gingival mucosa were encapsulated in modified fibrin hydrogels with different thicknesses and concentrations. Constructs with cells were subjected to a single-time exposure to red (630 nm) and near-infrared (IR) (840 nm) low-intensity irradiation. After 3 days of cultivation, the viability and physiological activity of the cells were analyzed using confocal microscopy and a set of classical tests for cytotoxicity. Result