Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect
Volkov A. V., Muraev A. A., Zharkova I. I., Voinova V. V., Akoulina E. A., Zhuikov V. A., Khaydapova D. D., Chesnokova D. V., Menshikh K. A., Dudun A. A., Makhina T. K., Bonartseva G. A., Asfarov T. F., Stamboliev I. A., Gazhva Y. V., Ryabova V. M., Zlatev L. H., Ivanov S. Yu., Shaitan K. V., Bonartsev A. P.
Materials Science and Engineering: C
Vol.114, Num.110991
Опубликовано: 2020
Тип ресурса: Статья
DOI:10.1016/j.msec.2020.110991
Аннотация:
A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials. In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats. The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing. To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison. The produced scaffolds have high porosity and irregular interconnected pore structure. PHB/HA scaffolds supported MSC growth and induced cell osteogenic different
Ключевые слова:
Alginate; Critical-sized bone defect; Hybrid scaffolds; Hydroxyapatite; Mesenchymal stem cells; Poly(3-hydroxybutyrate)
3D printers; Biocompatibility; Biodegradable polymers; Biopolymers; Bone; Cell culture; Computerized tomography; Defects; Filled polymers; Flowcharting; Fluorescence; Hydrogels; Hydroxyapatite; Pore structure; Rats; Stem cells; Tissue regeneration; Biocompatible polymer; Fluorescent microscopy; Interconnected pores; Intraperitoneal administration; Mesenchymal stem cell; Osteogenic differentiation; Osteoinductive properties; Poly(3-hydroxybutyrate); Scaffolds (biology)
Язык текста: Английский
ISSN: 1873-0191
Volkov A. V.
Muraev A. A.
Zharkova I. I.
Voinova V. V.
Akoulina E. A.
Zhuikov V. A.
Khaydapova D. D.
Chesnokova D. V.
Menshikh K. A.
Dudun A. A.
Makhina T. K.
Bonartseva G. A.
Asfarov T. F.
Stamboliev I. A.
Gazhva Y. V.
Ryabova V. M.
Zlatev L. H.
Ivanov S. Yu. Sergej Yuryevich 1957-
Shaitan K. V.
Bonartsev A. P.
Волков А. В.
Мураев А. А.
Жаркова И. И.
Воинова В. В.
Акоулина Е. А.
Жуиков В. А.
Хайдапова Д. Д.
Чеснокова Д. В.
Менших К. А.
Дудун А. А.
Махина Т. К.
Бонарцева Г. А.
Асфаров Т. Ф.
Стамболиев И. А.
Гажва Y. В.
Рябова В. М.
Златев Л. Х.
Иванов С. Ю. Сергей Юрьевич 1957-
Шаитан К. В.
Бонарцев А. П.
Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect
Текст визуальный непосредственный
Materials Science and Engineering: C
Elsevier Science Publisher B.V.
Vol.114 Num.110991
2020
Статья
Alginate Critical-sized bone defect Hybrid scaffolds Hydroxyapatite Mesenchymal stem cells Poly(3-hydroxybutyrate)
3D printers Biocompatibility Biodegradable polymers Biopolymers Bone Cell culture Computerized tomography Defects Filled polymers Flowcharting Fluorescence Hydrogels Hydroxyapatite Pore structure Rats Stem cells Tissue regeneration Biocompatible polymer Fluorescent microscopy Interconnected pores Intraperitoneal administration Mesenchymal stem cell Osteogenic differentiation Osteoinductive properties Poly(3-hydroxybutyrate) Scaffolds (biology)
A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials. In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats. The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing. To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison. The produced scaffolds have high porosity and irregular interconnected pore structure. PHB/HA scaffolds supported MSC growth and induced cell osteogenic different