Tracing upconversion nanoparticle penetration in human skin
Khabir Z., Guller A. E., Rozova V. S., Liang L., Lai Y. -., Goldys E. M., Hu H., Vickery K., Zvyagin A. V.
Colloids and Surfaces B: Biointerfaces
Vol.184, Num.110480
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.colsurfb.2019.110480
Аннотация:
Due to their unique optical properties upconversion nanoparticles (UCNPs) provide exceptionally high contrast for imaging of true nanoparticle distribution in excised human skin. It makes possible to show penetration of solid nanoparticles in skin treated with chemical enhancers. We demonstrated tracing upconversion nanoparticles in excised human skin by means of optical microscopy at the discrete particle level sensitivity to obtain their penetration profiles, which was validated by laser-ablation inductively-coupled-plasma mass-spectrometry. To demonstrate utilities of our method, UCNPs were coated with polymers, formulated in water and chemical enhancers, and applied on excised human skin mounted on Franz cells, followed by imaging using a custom-built laser-scanning microscope. To evaluate the toxicity impact on skin by polymer-coated UCNPs, we introduced a tissue engineering model of viable epidermis made of decellularized chick embryo skin seeded with keratinocytes. UCNPs formula
Ключевые слова:
Background-free optical imaging; Cytotoxicity; Penetration enhancer; Tissue engineering; Transdermal delivery; Upconversion nanoparticles
Cell culture; Cytotoxicity; Laser ablation; Mass spectrometry; Optical data storage; Optical microscopy; Optical properties; Plastic coatings; Polymers; Tissue; Tissue engineering; Ethanol-water solutions; Laser ablation inductively-coupled plasma mass spectrometries; Laser scanning microscope; Optical imaging; Penetration enhancers; Penetration profiles; Transdermal delivery; Upconversion nanoparticles; Nanoparticles; alcohol; cosmetic; penetration enhancing agent; polyethyleneimine; polymer; upconversion nanoparticle; water; biocompatible coated material; nanoparticle; nile red; oxazine derivative; polymer; animal cell; animal tissue; Article; chick; controlled study; cytotoxicity; decellularization; embryo; epidermis; HaCat cell line; human; human cell; human tissue; in vitro study; laser ablation inductively coupled plasma mass spectrometry; laser microscopy; microscopy; monolayer culture; nanotoxicology; nonhuman; priority journal; skin penetration; skin toxicity; stratum corneum
Язык текста: Английский
ISSN: 1873-4367
Khabir Z.
Guller A. E. Anna Evgenyevna 1973-
Rozova V. S.
Liang L.
Lai Y. -. Y.-J.
Goldys E. M.
Hu H.
Vickery K.
Zvyagin A. V. Andrej Vasilyevich 1961-
Хабир З.
Гуллер А. Е. Анна Евгеньевна 1973-
Розова В. С.
Лианг Л.
Лаи Y. -. Y.-Й.
Голдyс Е. М.
Ху Х.
Виcкерy К.
Звягин А. В. Андрей Васильевич 1961-
Tracing upconversion nanoparticle penetration in human skin
Текст визуальный непосредственный
Colloids and Surfaces B: Biointerfaces
Elsevier Science Publisher B.V.
Vol.184 Num.110480
2019
Статья
Background-free optical imaging Cytotoxicity Penetration enhancer Tissue engineering Transdermal delivery Upconversion nanoparticles
Cell culture Cytotoxicity Laser ablation Mass spectrometry Optical data storage Optical microscopy Optical properties Plastic coatings Polymers Tissue Tissue engineering Ethanol-water solutions Laser ablation inductively-coupled plasma mass spectrometries Laser scanning microscope Optical imaging Penetration enhancers Penetration profiles Transdermal delivery Upconversion nanoparticles Nanoparticles alcohol cosmetic penetration enhancing agent polyethyleneimine polymer upconversion nanoparticle water biocompatible coated material nanoparticle nile red oxazine derivative polymer animal cell animal tissue Article chick controlled study cytotoxicity decellularization embryo epidermis HaCat cell line human human cell human tissue in vitro study laser ablation inductively coupled plasma mass spectrometry laser microscopy microscopy monolayer culture nanotoxicology nonhuman priority journal skin penetration skin toxicity stratum corneum
Due to their unique optical properties upconversion nanoparticles (UCNPs) provide exceptionally high contrast for imaging of true nanoparticle distribution in excised human skin. It makes possible to show penetration of solid nanoparticles in skin treated with chemical enhancers. We demonstrated tracing upconversion nanoparticles in excised human skin by means of optical microscopy at the discrete particle level sensitivity to obtain their penetration profiles, which was validated by laser-ablation inductively-coupled-plasma mass-spectrometry. To demonstrate utilities of our method, UCNPs were coated with polymers, formulated in water and chemical enhancers, and applied on excised human skin mounted on Franz cells, followed by imaging using a custom-built laser-scanning microscope. To evaluate the toxicity impact on skin by polymer-coated UCNPs, we introduced a tissue engineering model of viable epidermis made of decellularized chick embryo skin seeded with keratinocytes. UCNPs formula