Efficiency of human olfactory ensheathing cell transplantation into spinal cysts to improve mobility of the hind limbs
Stepanova O. V., Voronova A. D., Chadin A. V., Valikhov M. P., Semkina A. S., Karsuntseva E. K., Chekhonin I. V., Shishkina V. S., Reshetov I. V., Chekhonin V. P.
Stem Cells and Development
Vol.28, Issue18, P. 1253-1263
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1089/scd.2019.0092
Аннотация:
The pathological processes developing after spinal cord injuries often lead to formation of cysts. Existing surgical and medical methods are insufficient for treatment of post-traumatic spinal cord cysts. One of the emerging tools is cell therapy. Olfactory ensheathing cells (OECs) are perspective cells for cell therapy. In this study, we demonstrated that human OEC transplantation is effective in experimental spinal cysts. For our experiments, we selected animals only at the intermediate stage of recovery with scores from 8 to 13 according to the Basso, Beattie, and Bresnahan (BBB) scale. Cells were transplanted in different quantities (0.75 and 1.5 million) into the fully formed cysts and in the areas of injury without cysts. Improvement of limb mobility after human OEC transplantation into post-traumatic cysts was shown. In the group of rats with cysts, time-dependent increase in the BBB score was observed in subgroups treated with 0.75 and 1.5 million OECs with no statistically sig
Ключевые слова:
cell therapy; human olfactory ensheathing cells; injury of spinal cord; olfactory mucosa; spinal cysts
animal experiment; animal model; animal tissue; Article; basso beattie and bresnahan scale; cell migration; cell transplantation; controlled study; energy recovery; female; general condition improvement; hindlimb; human; human cell; human tissue; Kruskal Wallis test; limb movement; nonhuman; olfactory ensheathing cell; olfactory mucosa; priority journal; rat; scoring system; spinal cord cyst; Wistar rat; animal; cell culture; cytology; mucosa; pathology; procedures; Schwann cell; spinal cord injury; stem cell transplantation; transplantation; Animals; Cells, Cultured; Female; Humans; Mucous Membrane; Olfactory Mucosa; Rats; Rats, Wistar; Schwann Cells; Spinal Cord Injuries; Stem Cell Transplantation
Язык текста: Английский
ISSN: 1557-8534
Stepanova O. V.
Voronova A. D.
Chadin A. V.
Valikhov M. P.
Semkina A. S.
Karsuntseva E. K.
Chekhonin I. V.
Shishkina V. S.
Reshetov I. V. Igor` Vladimirovich 1964-
Chekhonin V. P.
Степанова О. В.
Воронова А. Д.
Чадин А. В.
Валихов М. П.
Семкина А. С.
Карсунцева Е. К.
Чехонин И. В.
Шишкина В. С.
Решетов И. В. Игорь Владимирович 1964-
Чехонин В. П.
Efficiency of human olfactory ensheathing cell transplantation into spinal cysts to improve mobility of the hind limbs
Текст визуальный непосредственный
Stem Cells and Development
Mary Ann Liebert Inc.
Vol.28, Issue18 P. 1253-1263
2019
Статья
cell therapy human olfactory ensheathing cells injury of spinal cord olfactory mucosa spinal cysts
animal experiment animal model animal tissue Article basso beattie and bresnahan scale cell migration cell transplantation controlled study energy recovery female general condition improvement hindlimb human human cell human tissue Kruskal Wallis test limb movement nonhuman olfactory ensheathing cell olfactory mucosa priority journal rat scoring system spinal cord cyst Wistar rat animal cell culture cytology mucosa pathology procedures Schwann cell spinal cord injury stem cell transplantation transplantation Animals Cells, Cultured Female Humans Mucous Membrane Olfactory Mucosa Rats Rats, Wistar Schwann Cells Spinal Cord Injuries Stem Cell Transplantation
The pathological processes developing after spinal cord injuries often lead to formation of cysts. Existing surgical and medical methods are insufficient for treatment of post-traumatic spinal cord cysts. One of the emerging tools is cell therapy. Olfactory ensheathing cells (OECs) are perspective cells for cell therapy. In this study, we demonstrated that human OEC transplantation is effective in experimental spinal cysts. For our experiments, we selected animals only at the intermediate stage of recovery with scores from 8 to 13 according to the Basso, Beattie, and Bresnahan (BBB) scale. Cells were transplanted in different quantities (0.75 and 1.5 million) into the fully formed cysts and in the areas of injury without cysts. Improvement of limb mobility after human OEC transplantation into post-traumatic cysts was shown. In the group of rats with cysts, time-dependent increase in the BBB score was observed in subgroups treated with 0.75 and 1.5 million OECs with no statistically sig