Аннотация:
Neurovirulent enterovirus 71 (EV-A71) caused a massive epidemic in China in 2008-2011. While subgenotype C4 was the major causative agent, a few isolates were almost identical to the prototype EV-A71 strain and belonged to genotype A. This variant was allegedly extinct since 1970, and its identification in this epidemic suggests reintroduction of the archive virus. Regression analysis of genetic distances (TempEst software) was of moderate utility due to the low resolution of classical phylogenetic methods. Bayesian phylogenetic analysis (BEAST software) suggested artificial introduction event based on highly aberrant phylogenetic tree branch rates that differed by over three standard deviations from the mean substitution rate for EV71. Manual nucleotide-level analysis was used to further explore the virus spread pattern after introduction into circulation. Upon reintroduction, the virus accumulated up to seven substitutions in VP1, most of them non-synonymous and located within the ca
Ключевые слова:
Enterovirus; Enterovirus 71 (EV-A71); Laboratory escape; Molecular epidemiology
amino acid sequence; Article; DNA polymorphism; Enterovirus A71; gene mutation; gene sequence; genetic distance; genotype; molecular epidemiology; nonhuman; phylogeny; sequence analysis; species reintroduction; virus capsid; virus isolation; chemistry; China; classification; Enterovirus; Enterovirus A; Enterovirus infection; epidemic; genetics; human; isolation and purification; molecular model; mutation; phylogeography; virology; capsid protein; virus RNA; Capsid Proteins; China; Enterovirus; Enterovirus A, Human; Enterovirus Infections; Epidemics; Genotype; Humans; Models, Molecular; Molecular Epidemiology; Mutation; Phylogeny; Phylogeography; RNA, Viral
Vakulenko Yu. A. Yuliya Aleksandrovna 1994-
Devyatkin A. A. Andrej Andreevich 1990-
Lukashev A. N. Aleksandr Nikolaevich 1977-
Вакуленко Ю. А. Юлия Александровна 1994-
Девяткин А. А. Андрей Андреевич 1990-
Лукашев А. Н. Александр Николаевич 1977-
Using statistical phylogenetics for investigation of enterovirus 71 genotype a reintroduction into circulation
Текст визуальный непосредственный
Viruses
Vol.11, Issue10 Num.895
2019
Статья
Enterovirus Enterovirus 71 (EV-A71) Laboratory escape Molecular epidemiology
amino acid sequence Article DNA polymorphism Enterovirus A71 gene mutation gene sequence genetic distance genotype molecular epidemiology nonhuman phylogeny sequence analysis species reintroduction virus capsid virus isolation chemistry China classification Enterovirus Enterovirus A Enterovirus infection epidemic genetics human isolation and purification molecular model mutation phylogeography virology capsid protein virus RNA Capsid Proteins China Enterovirus Enterovirus A, Human Enterovirus Infections Epidemics Genotype Humans Models, Molecular Molecular Epidemiology Mutation Phylogeny Phylogeography RNA, Viral
Neurovirulent enterovirus 71 (EV-A71) caused a massive epidemic in China in 2008-2011. While subgenotype C4 was the major causative agent, a few isolates were almost identical to the prototype EV-A71 strain and belonged to genotype A. This variant was allegedly extinct since 1970, and its identification in this epidemic suggests reintroduction of the archive virus. Regression analysis of genetic distances (TempEst software) was of moderate utility due to the low resolution of classical phylogenetic methods. Bayesian phylogenetic analysis (BEAST software) suggested artificial introduction event based on highly aberrant phylogenetic tree branch rates that differed by over three standard deviations from the mean substitution rate for EV71. Manual nucleotide-level analysis was used to further explore the virus spread pattern after introduction into circulation. Upon reintroduction, the virus accumulated up to seven substitutions in VP1, most of them non-synonymous and located within the ca