Molecular genetic mechanisms of influence of laser radiation with 577 nm wavelength in a microimpulse mode on the condition of the retina
Gavrilova N. A., Borzenok S. A., Zaletaev D. V., Solomin V. A., Gadzhieva N. S., Tishchenko O. E., Komova O. U., Zinov'eva A. V.
Experimental Eye Research
Vol.185, Num.107650
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.exer.2019.04.018
Аннотация:
The aim of the study: was to investigate the molecular genetic mechanisms of the influence of laser radiation with 577 nm wavelength in a microimpulse mode on the retina in the experimental conditions after the intravitreal injection of VEGF. Materials and methods: The study was performed on 4-5 week-old male mice of the line C57BL/6J. The animals were divided into 4 groups of 5 mice in each group, one eye was excremental, the contralateral eye remained intact. In the first group, intravitreal injection of PBS was performed; in the second group, intravitreal injection of 50 ng/ml of recombinant VEGF165 in 2 μL of phosphate-buffered saline (PBS) was performed; in the third and fourth groups, a day after the intravitreal injection of recombinant VEGF165, laser radiation with wavelength 577 nm was applied in the micropulse and continuous modes, respectively. Tissue samples (neuroepithelium, pigment epithelium) for the microarray transcription analysis in the animals from group 1 and 2 wer
Ключевые слова:
Genes expression; Microimpulse laser radiation; Retina; Vascular endothelial growth factor (VEGF)
aldehyde dehydrogenase; ankyrin; basic leucine zipper transcription factor; carnitine palmitoyltransferase; cyclin dependent kinase inhibitor 2C; cysteine dioxygenase; cytochrome P450 family 27; cytokeratin 13; cytokeratin 14; cytokeratin 15; cytokeratin 17; cytokeratin 19; glycoprotein; histone; keratin; keratin 23; keratin 6b; lamin A; lysozyme; mucin 1; myeloid differentiation factor 88; nuclear protein; prodynorphin; protein kinase C beta; somatomedin binding protein 4; stefin; ubiquilin 1; unclassified drug; uridine phosphorylase; vasculotropin 165; very low density lipoprotein receptor; eye protein; glycosylphosphatidylinositol anchored protein; Gp2 protein, mouse; membrane protein; recombinant protein; Rhbdf1 protein, mouse; somatomedin binding protein 4; uridine phosphorylase; vasculotropin A; aldh3a1 gene; aldoc gene; animal cell; animal experiment; animal model; animal tissue; ankrd23 gene; anp32a gene; Article; axon; cell differentiation; cell proliferation; controlled study
Язык текста: Английский
ISSN: 1096-0007
Gavrilova N. A.
Borzenok S. A.
Zaletaev D. V. Dmitrij Vladimirovich 1956-
Solomin V. A.
Gadzhieva N. S.
Tishchenko O. E.
Komova O. U.
Zinov'eva A. V.
Гаврилова Н. А.
Борзенок С. А.
Залетаев Д. В. Дмитрий Владимирович 1956-
Соломин В. А.
Гаджиева Н. С.
Тишченко О. Е.
Комова О. У.
Зинов'ева А. В.
Molecular genetic mechanisms of influence of laser radiation with 577 nm wavelength in a microimpulse mode on the condition of the retina
Текст визуальный непосредственный
Experimental Eye Research
Academic Press
Vol.185 Num.107650
2019
Статья
Genes expression Microimpulse laser radiation Retina Vascular endothelial growth factor (VEGF)
aldehyde dehydrogenase ankyrin basic leucine zipper transcription factor carnitine palmitoyltransferase cyclin dependent kinase inhibitor 2C cysteine dioxygenase cytochrome P450 family 27 cytokeratin 13 cytokeratin 14 cytokeratin 15 cytokeratin 17 cytokeratin 19 glycoprotein histone keratin keratin 23 keratin 6b lamin A lysozyme mucin 1 myeloid differentiation factor 88 nuclear protein prodynorphin protein kinase C beta somatomedin binding protein 4 stefin ubiquilin 1 unclassified drug uridine phosphorylase vasculotropin 165 very low density lipoprotein receptor eye protein glycosylphosphatidylinositol anchored protein Gp2 protein, mouse membrane protein recombinant protein Rhbdf1 protein, mouse somatomedin binding protein 4 uridine phosphorylase vasculotropin A aldh3a1 gene aldoc gene animal cell animal experiment animal model animal tissue ankrd23 gene anp32a gene Article axon cell differentiation cell proliferation controlled study
The aim of the study: was to investigate the molecular genetic mechanisms of the influence of laser radiation with 577 nm wavelength in a microimpulse mode on the retina in the experimental conditions after the intravitreal injection of VEGF. Materials and methods: The study was performed on 4-5 week-old male mice of the line C57BL/6J. The animals were divided into 4 groups of 5 mice in each group, one eye was excremental, the contralateral eye remained intact. In the first group, intravitreal injection of PBS was performed; in the second group, intravitreal injection of 50 ng/ml of recombinant VEGF165 in 2 μL of phosphate-buffered saline (PBS) was performed; in the third and fourth groups, a day after the intravitreal injection of recombinant VEGF165, laser radiation with wavelength 577 nm was applied in the micropulse and continuous modes, respectively. Tissue samples (neuroepithelium, pigment epithelium) for the microarray transcription analysis in the animals from group 1 and 2 wer