Medicinal leech antimicrobial peptides lacking toxicity represent a promising alternative strategy to combat antibiotic-resistant pathogens
Grafskaia E. N., Nadezhdin K. D., Talyzina I. A., Polina N. F., Podgorny O. V., Pavlova E. R., Bashkirov P. V., Kharlampieva D. D., Bobrovsky P. A., Latsis I. A., Manuvera V. A., Babenko V. V., Trukhan V. M., Arseniev A. S., Klinov D. V., Lazarev V. N.
European Journal of Medicinal Chemistry
Vol.180, P. 143-153
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.ejmech.2019.06.080
Аннотация:
The rise of antibiotic resistance has necessitated the development of alternative strategies for the treatment of infectious diseases. Antimicrobial peptides (AMPs), components of the innate immune response in various organisms, are promising next-generation drugs against bacterial infections. The ability of the medicinal leech Hirudo medicinalis to store blood for months with little change has attracted interest regarding the identification of novel AMPs in this organism. In this study, we employed computational algorithms to the medicinal leech genome assembly to identify amino acid sequences encoding potential AMPs. Then, we synthesized twelve candidate AMPs identified by the algorithms, determined their secondary structures, measured minimal inhibitory concentrations against three bacterial species (Escherichia coli, Bacillus subtilis, and Chlamydia thrachomatis), and assayed cytotoxic and haemolytic activities. Eight of twelve candidate AMPs possessed antimicrobial activity, and o
Ключевые слова:
Antimicrobial peptides; Genome; Hirudo medicinalis; Medicinal leech; Prediction algorithm
melittin; polypeptide antibiotic agent; antiinfective agent; antimicrobial cationic peptide; algorithm; amino acid sequence; antibiotic resistance; antimicrobial activity; Article; Bacillus subtilis; bacterial cell; bacterial membrane; broth dilution; carboxy terminal sequence; cell survival; cell viability; comparative study; controlled study; cytotoxicity; drug synthesis; Escherichia coli; hemolysis; heteronuclear single quantum coherence; Hirudo medicinalis; human; human cell; hydrophobicity; lipid vesicle; McCoy cell line; minimum inhibitory concentration; nonhuman; protein secondary structure; scanning electron microscopy; animal; antibiotic resistance; bacterial infection; cell line; chemical structure; chemistry; Chlamydia; dose response; drug effect; growth, development and aging; Hirudo medicinalis; structure activity relation; Algorithms; Animals; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Bacillus subtilis; Bacterial Infections; Cell Line; Cell Survival; Chlamyd
Язык текста: Английский
ISSN: 1768-3254
Grafskaia E. N.
Nadezhdin K. D.
Talyzina I. A.
Polina N. F.
Podgorny O. V.
Pavlova E. R.
Bashkirov P. V.
Kharlampieva D. D.
Bobrovsky P. A.
Latsis I. A.
Manuvera V. A.
Babenko V. V.
Trukhan V. M. Vladimir Mikhaylovich 1971-
Arseniev A. S.
Klinov D. V.
Lazarev V. N.
Графскаиа Е. Н.
Надеждин К. Д.
Талyзина И. А.
Полина Н. Ф.
Подгорнy О. В.
Павлова Е. Р.
Башкиров П. В.
Харлампиева Д. Д.
Бобровскy П. А.
Лацис И. А.
Манувера В. А.
Бабенко В. В.
Трухан В. М. Владимир Михайлович 1971-
Арсениев А. С.
Клинов Д. В.
Лазарев В. Н.
Medicinal leech antimicrobial peptides lacking toxicity represent a promising alternative strategy to combat antibiotic-resistant pathogens
Текст визуальный непосредственный
European Journal of Medicinal Chemistry
Elsevier Science Publisher B.V.
Vol.180 P. 143-153
2019
Статья
Antimicrobial peptides Genome Hirudo medicinalis Medicinal leech Prediction algorithm
melittin polypeptide antibiotic agent antiinfective agent antimicrobial cationic peptide algorithm amino acid sequence antibiotic resistance antimicrobial activity Article Bacillus subtilis bacterial cell bacterial membrane broth dilution carboxy terminal sequence cell survival cell viability comparative study controlled study cytotoxicity drug synthesis Escherichia coli hemolysis heteronuclear single quantum coherence Hirudo medicinalis human human cell hydrophobicity lipid vesicle McCoy cell line minimum inhibitory concentration nonhuman protein secondary structure scanning electron microscopy animal antibiotic resistance bacterial infection cell line chemical structure chemistry Chlamydia dose response drug effect growth, development and aging Hirudo medicinalis structure activity relation Algorithms Animals Anti-Bacterial Agents Antimicrobial Cationic Peptides Bacillus subtilis Bacterial Infections Cell Line Cell Survival Chlamyd
The rise of antibiotic resistance has necessitated the development of alternative strategies for the treatment of infectious diseases. Antimicrobial peptides (AMPs), components of the innate immune response in various organisms, are promising next-generation drugs against bacterial infections. The ability of the medicinal leech Hirudo medicinalis to store blood for months with little change has attracted interest regarding the identification of novel AMPs in this organism. In this study, we employed computational algorithms to the medicinal leech genome assembly to identify amino acid sequences encoding potential AMPs. Then, we synthesized twelve candidate AMPs identified by the algorithms, determined their secondary structures, measured minimal inhibitory concentrations against three bacterial species (Escherichia coli, Bacillus subtilis, and Chlamydia thrachomatis), and assayed cytotoxic and haemolytic activities. Eight of twelve candidate AMPs possessed antimicrobial activity, and o