Influence of auto-and xenogenic fibroblastes and dermal equivalent on macrophage content in granulative tissue of ishemic cutaneus wound on the 12...
Шаповалова Е. Y., Демяшкин Г. А., Боyко Т. А., Барановскиy Y. Г., Морозова М. Н., Барановскиy А. Г., Агеева Е. С.
Медицинский вестник Северного Кавказа
Т. 14, Вып. 1, С. 255-260
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.14300/mnnc.2019.14028
Аннотация:
The presence of macrophages in the tissues of the regenerating model of skin ischemic wounds was determined on the mature C57/B1 mice after administration of auto- and xenogenic fibroblasts, and also after transplantation of the dermal equivalent with xenogenic fibroblasts. Macrophages were identified by the presence of the CD68 antigen by monoclonal antibodies by immunohistochemistry. On the 12th day of healing the experimental ischemic wound, the histological structure of the biopsy specimen after cutting the wound with auto- and xenogenic fibroblasts on the DMEM F12 growth medium and after transplantation of the dermal equivalent with xenogenic differ from the control by the level of development of the epidermis and statistically significant decrease in the macrophage index. The results obtained indicate a statistically significant reduction in the duration of inflammation and earlier and active formation of granulation tissue when using autofibroblasts. © 2019 Stavropol State Medic
Ключевые слова:
Cellular technology; Dermal equivalent; Fibroblast; Macrophage; Skin; Wound healing
CD68 antigen; animal cell; animal experiment; animal model; animal tissue; Article; fibroblast; granulation tissue; histogenesis; immunohistochemistry; inflammation; macrophage; mouse; nonhuman; skin biopsy; skin injury; transplantation; wound healing
Язык текста: Русский
ISSN: 2073-8137
Шаповалова Е. Y.
Демяшкин Г. А. Григорий Александрович 1976-
Боyко Т. А.
Барановскиy Y. Г.
Морозова М. Н.
Барановскиy А. Г.
Агеева Е. С.
Shapovalova E. Y.
Demyashkin G. A. Grigorij Aleksandrovich 1976-
Boyko T. A.
Baranovskiy Y. G.
Morozova M. N.
Baranovskiy A. G.
Ageeva E. S.
Influence of auto-and xenogenic fibroblastes and dermal equivalent on macrophage content in granulative tissue of ishemic cutaneus wound on the 12 day of regenerative histogenesis
Influence of auto-and xenogenic fibroblastes and dermal equivalent on macrophage content in granulative tissue of ishemic cutaneus wound on the 12...
Текст визуальный непосредственный
Медицинский вестник Северного Кавказа
Ставропольский государственный медицинский университет
Т. 14, Вып. 1 С. 255-260
2019
Статья
Cellular technology Dermal equivalent Fibroblast Macrophage Skin Wound healing
CD68 antigen animal cell animal experiment animal model animal tissue Article fibroblast granulation tissue histogenesis immunohistochemistry inflammation macrophage mouse nonhuman skin biopsy skin injury transplantation wound healing
The presence of macrophages in the tissues of the regenerating model of skin ischemic wounds was determined on the mature C57/B1 mice after administration of auto- and xenogenic fibroblasts, and also after transplantation of the dermal equivalent with xenogenic fibroblasts. Macrophages were identified by the presence of the CD68 antigen by monoclonal antibodies by immunohistochemistry. On the 12th day of healing the experimental ischemic wound, the histological structure of the biopsy specimen after cutting the wound with auto- and xenogenic fibroblasts on the DMEM F12 growth medium and after transplantation of the dermal equivalent with xenogenic differ from the control by the level of development of the epidermis and statistically significant decrease in the macrophage index. The results obtained indicate a statistically significant reduction in the duration of inflammation and earlier and active formation of granulation tissue when using autofibroblasts. © 2019 Stavropol State Medic