The cardioprotective effects of diallyl trisulfide on diabetic rats with ex vivo induced ischemia/reperfusion injury
Jeremic J. N., Yakovlevich V., Zivkovic V. I., Srejovic I. M., Bradic J. V., Bolevich S., Nikolic T. T. R., Mitrovic S. L., Jovicic N. U., Tyagi S. C., Jeremic N. S.
Molecular and Cellular Biochemistry
Vol.460, Issue1-2, P. 151-164
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s11010-019-03577-w
Аннотация:
Diallyl trisulfide (DATS) is distinguished as the most potent polysulfide isolated from garlic. The aim of our study was to investigate effects of oral administration of DATS on healthy and diabetic rats, with special attention on heart function. Rats were randomly divided into four groups: CTRL (healthy rats), DATS (healthy rats treated with DATS), DM (diabetic rats), DM + DATS (diabetic rats treated with DATS). DATS (40 mg/kg of body weight) was administered every other day for 3 weeks, at the end of which rats underwent echocardiography, glycemic measurement and redox status assessment. Isolated rat hearts were subjected to 30 min global ischemia and 60 min reperfusion, after which heart tissue was counterstain with hematoxylin and eosin and cardiac Troponin T staining (cTnT), while expression of Bax, B cell lymphoma 2 (Bcl-2), caspase-3, caspase-9 and superoxide dismutase-2 were examined in the left ventricle. DATS treatment significantly reduced blood glucose levels of diabetic ra
Ключевые слова:
Cardioprotection; Diabetes; Diallyl trisulfide; Ischemia–reperfusion injury; Isolated heart
beta actin; caspase 3; caspase 9; diallyl trisulfide; manganese superoxide dismutase; protein Bax; protein bcl 2; troponin T; allyl compound; biological marker; cardiotonic agent; diallyl trisulfide; sulfide; animal cell; animal experiment; animal model; animal tissue; apoptosis; Article; biochemical analysis; body weight; cardiac muscle; diabetes mellitus; echocardiography; ex vivo study; fluid intake; food intake; gene expression; glucose blood level; glucose level; heart; heart function; heart left ventricle contractility; heart muscle contractility; heart muscle injury; heart muscle reperfusion; heart protection; heart tissue; heart ventricle remodeling; heart weight; immunohistochemistry; in vivo study; infant; male; nonhuman; oxidation; oxidative stress; rat; reperfusion injury; staining; Wistar rat; animal; complication; drug effect; experimental diabetes mellitus; metabolism; pathology; pathophysiology; reperfusion injury; Allyl Compounds; Animals; Apoptosis; Biomarkers; Cardio
Язык текста: Английский
ISSN: 1573-4919
Jeremic J. N.
Yakovlevich V. Vladimir 1971-
Zivkovic V. I.
Srejovic I. M.
Bradic J. V.
Bolevich S. Sergej 1964-
Nikolic T. T. R. Turnic T.R.
Mitrovic S. L.
Jovicic N. U.
Tyagi S. C.
Jeremic N. S.
Йеремиc Й. Н.
Яковлевич В. Владимир 1971-
Зивковиc В. И.
Срейовиc И. М.
Брадиc Й. В.
Болевич С. Сергей 1964-
Николиc Т. Т. Р. Тюрниc Т.Р.
Митровиc С. Л.
Йовиcиc Н. У.
Тяги С. C.
Йеремиc Н. С.
The cardioprotective effects of diallyl trisulfide on diabetic rats with ex vivo induced ischemia/reperfusion injury
Текст визуальный непосредственный
Molecular and Cellular Biochemistry
Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Vol.460, Issue1-2 P. 151-164
2019
Статья
Cardioprotection Diabetes Diallyl trisulfide Ischemia–reperfusion injury Isolated heart
beta actin caspase 3 caspase 9 diallyl trisulfide manganese superoxide dismutase protein Bax protein bcl 2 troponin T allyl compound biological marker cardiotonic agent diallyl trisulfide sulfide animal cell animal experiment animal model animal tissue apoptosis Article biochemical analysis body weight cardiac muscle diabetes mellitus echocardiography ex vivo study fluid intake food intake gene expression glucose blood level glucose level heart heart function heart left ventricle contractility heart muscle contractility heart muscle injury heart muscle reperfusion heart protection heart tissue heart ventricle remodeling heart weight immunohistochemistry in vivo study infant male nonhuman oxidation oxidative stress rat reperfusion injury staining Wistar rat animal complication drug effect experimental diabetes mellitus metabolism pathology pathophysiology reperfusion injury Allyl Compounds Animals Apoptosis Biomarkers Cardio
Diallyl trisulfide (DATS) is distinguished as the most potent polysulfide isolated from garlic. The aim of our study was to investigate effects of oral administration of DATS on healthy and diabetic rats, with special attention on heart function. Rats were randomly divided into four groups: CTRL (healthy rats), DATS (healthy rats treated with DATS), DM (diabetic rats), DM + DATS (diabetic rats treated with DATS). DATS (40 mg/kg of body weight) was administered every other day for 3 weeks, at the end of which rats underwent echocardiography, glycemic measurement and redox status assessment. Isolated rat hearts were subjected to 30 min global ischemia and 60 min reperfusion, after which heart tissue was counterstain with hematoxylin and eosin and cardiac Troponin T staining (cTnT), while expression of Bax, B cell lymphoma 2 (Bcl-2), caspase-3, caspase-9 and superoxide dismutase-2 were examined in the left ventricle. DATS treatment significantly reduced blood glucose levels of diabetic ra