Quantitative detection of double bonds in hyaluronic acid derivative via permanganate ion reduction
Sochilina A. V., Savelyev A. G., Demina P. A., Sizova S. V., Zubov V. P., Khaydukov E. V., Generalova A. N.
Measurement Science and Technology
Vol.30, Issue7, Num.075102
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1088/1361-6501/ab0fb4
Аннотация:
The detection of double bonds in polymers exploited as scaffold materials, e.g. hyaluronic acid (HA) derivatives, is crucial for their further applications. The quantity variations of unsaturated units significantly affect the photoinduced crosslinking degree responsible for the mechanical properties of scaffolds. We demonstrate a simple, selective and rapid quantitative method that enables the detection of double bond moieties in polymers. The approach is based on a reaction with potassium permanganate which is spectrophotometrically confirmed. The effect of glycidyl methacrylate concentration and various reaction catalysts introduced at the stage of HA modification, as well as the change of double bond concentration over time, has been investigated using the proposed method. In addition, the developed quantitative method can be utilized for rapid and efficient detection of unreacted double bonds after photopolymerization as a tool for monitoring conversion over time. © 2019 IOP Publi
Ключевые слова:
double bonds; evaluation studies; hyaluronic acid derivatives; potassium permanganate
Acrylic monomers; Mechanical properties; Organic acids; Photopolymerization; Potash; Scaffolds; Double bonds; Evaluation study; Glycidyl methacrylate; Hyaluronic acid derivatives; Photo-induced crosslinking; Potassium permanganate; Quantitative detection; Quantitative method; Hyaluronic acid
Язык текста: Английский
ISSN: 1361-6501
Sochilina A. V.
Savelyev A. G. Aleksandr Georgievich 1986-
Demina P. A.
Sizova S. V.
Zubov V. P.
Khaydukov E. V. Evgenij Valeryevich 1984-
Generalova A. N.
Сочилина А. В.
Савельев А. Г. Александр Георгиевич 1986-
Демина П. А.
Сизова С. В.
Зубов В. П.
Хайдуков Е. В. Евгений Валерьевич 1984-
Генералова А. Н.
Quantitative detection of double bonds in hyaluronic acid derivative via permanganate ion reduction
Текст визуальный непосредственный
Measurement Science and Technology
Institute of Physics and IOP Publishing Limited
Vol.30, Issue7 Num.075102
2019
Статья
double bonds evaluation studies hyaluronic acid derivatives potassium permanganate
Acrylic monomers Mechanical properties Organic acids Photopolymerization Potash Scaffolds Double bonds Evaluation study Glycidyl methacrylate Hyaluronic acid derivatives Photo-induced crosslinking Potassium permanganate Quantitative detection Quantitative method Hyaluronic acid
The detection of double bonds in polymers exploited as scaffold materials, e.g. hyaluronic acid (HA) derivatives, is crucial for their further applications. The quantity variations of unsaturated units significantly affect the photoinduced crosslinking degree responsible for the mechanical properties of scaffolds. We demonstrate a simple, selective and rapid quantitative method that enables the detection of double bond moieties in polymers. The approach is based on a reaction with potassium permanganate which is spectrophotometrically confirmed. The effect of glycidyl methacrylate concentration and various reaction catalysts introduced at the stage of HA modification, as well as the change of double bond concentration over time, has been investigated using the proposed method. In addition, the developed quantitative method can be utilized for rapid and efficient detection of unreacted double bonds after photopolymerization as a tool for monitoring conversion over time. © 2019 IOP Publi