Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ
Ptushenko V. V., Solovchenko A. E., Bychkov A. Y., Chivkunova O. B., Golovin A. V., Gorelova O. A., Ismagulova T. T., Kulik L. V., Lobakova E. S., Lukyanov A. A., Samoilova R. I., Scherbakov P. N., Selyakh I. O., Semenova L. R., Vasilieva S. G., Baulina O. I., Skulachev M. V., Kirpichnikov M. P.
Photosynthesis Research
Vol.142, Issue2, P. 229-240
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s11120-019-00657-2
Аннотация:
Mitochondria-targeted antioxidants (also known as ‘Skulachev Ions’ electrophoretically accumulated by mitochondria) exert anti-ageing and ROS-protecting effects well documented in animal and human cells. However, their effects on chloroplast in photosynthetic cells and corresponding mechanisms are scarcely known. For the first time, we describe a dramatic quenching effect of (10-(6-plastoquinonyl)decyl triphenylphosphonium (SkQ1) on chlorophyll fluorescence, apparently mediated by redox interaction of SkQ1 with Mn cluster in Photosystem II (PSII) of chlorophyte microalga Chlorella vulgaris and disabling the oxygen-evolving complex (OEC). Microalgal cells displayed a vigorous uptake of SkQ1 which internal concentration built up to a very high level. Using optical and EPR spectroscopy, as well as electron donors and in silico molecular simulation techniques, we found that SkQ1 molecule can interact with Mn atoms of the OEC in PSII. This stops water splitting giving rise to potent quenche
Ключевые слова:
Chlorophyll fluorescence quenching; Oxygen-evolving complex; P680 + accumulation; Photosystem II; Skulachev ions; YZ
10-(6'-plastoquinonyl)decyltriphenylphosphonium; antioxidant; cation; chlorophyll; manganese; oxygen; plastoquinone; chemical phenomena; Chlorella vulgaris; drug effect; fluorescence; kinetics; light; metabolism; molecular docking; photosystem II; Antioxidants; Cations; Chlorella vulgaris; Chlorophyll; Fluorescence; Hydrophobic and Hydrophilic Interactions; Kinetics; Light; Manganese; Molecular Docking Simulation; Oxygen; Photosystem II Protein Complex; Plastoquinone
Язык текста: Английский
ISSN: 1573-5079
Ptushenko V. V.
Solovchenko A. E.
Bychkov A. Y.
Chivkunova O. B.
Golovin A. V. Andrej Viktorovich 1975-
Gorelova O. A.
Ismagulova T. T.
Kulik L. V.
Lobakova E. S.
Lukyanov A. A.
Samoilova R. I.
Scherbakov P. N.
Selyakh I. O.
Semenova L. R.
Vasilieva S. G.
Baulina O. I.
Skulachev M. V.
Kirpichnikov M. P.
Птюшенко В. В.
Соловченко А. Е.
Бyчков А. Y.
Чивкунова О. Б.
Головин А. В. Андрей Викторович 1975-
Горелова О. А.
Исмагулова Т. Т.
Кулик Л. В.
Лобакова Е. С.
Лукянов А. А.
Самоилова Р. И.
Счербаков П. Н.
Селях И. О.
Семенова Л. Р.
Василиева С. Г.
Баулина О. И.
Скулачев М. В.
Кирпичников М. П.
Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ
Текст визуальный непосредственный
Photosynthesis Research
Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Vol.142, Issue2 P. 229-240
2019
Статья
Chlorophyll fluorescence quenching Oxygen-evolving complex P680 + accumulation Photosystem II Skulachev ions YZ
10-(6'-plastoquinonyl)decyltriphenylphosphonium antioxidant cation chlorophyll manganese oxygen plastoquinone chemical phenomena Chlorella vulgaris drug effect fluorescence kinetics light metabolism molecular docking photosystem II Antioxidants Cations Chlorella vulgaris Chlorophyll Fluorescence Hydrophobic and Hydrophilic Interactions Kinetics Light Manganese Molecular Docking Simulation Oxygen Photosystem II Protein Complex Plastoquinone
Mitochondria-targeted antioxidants (also known as ‘Skulachev Ions’ electrophoretically accumulated by mitochondria) exert anti-ageing and ROS-protecting effects well documented in animal and human cells. However, their effects on chloroplast in photosynthetic cells and corresponding mechanisms are scarcely known. For the first time, we describe a dramatic quenching effect of (10-(6-plastoquinonyl)decyl triphenylphosphonium (SkQ1) on chlorophyll fluorescence, apparently mediated by redox interaction of SkQ1 with Mn cluster in Photosystem II (PSII) of chlorophyte microalga Chlorella vulgaris and disabling the oxygen-evolving complex (OEC). Microalgal cells displayed a vigorous uptake of SkQ1 which internal concentration built up to a very high level. Using optical and EPR spectroscopy, as well as electron donors and in silico molecular simulation techniques, we found that SkQ1 molecule can interact with Mn atoms of the OEC in PSII. This stops water splitting giving rise to potent quenche