Аннотация:
The objective of the research was to elaborate experimental-theoretical and clinic-bacteriological rationale for the application of laser diagnostic for identification of main pathogens of purulent-inflammatory processes in maxillofacial area. For germs identification by giant Raman scattering effect SERS-substrate with nano silver metallic balls, reference strains (Ps. aeruginosa 27853 and S. aureus 25923) and clinical cultures of Staphylococcus, Bacillus and Escherichia coli were used. Using an example of purulent inflammation pathogens we considered that each of bacterial species is characterized by individual spectral lines of Raman scattering, which allows to identify them in short term (1-2 min). Moreover the proposed method is highly sensitive (105-106 CFU/ml). Creation of germs library and device portability makes use of laser diagnostic for express-indication purulent infections possible directly in clinical conditions. Thus, analytical capability, quick result, high sensitivi
Ключевые слова:
antibiotic-resistance; clinical microbiology; microorganism indication; raman peaks; raman-spectrum library; SERS-substrates; suppurative surgical infection
colloidal silver; metal nanoparticle; silver; bacterium; classification; devices; face; human; isolation and purification; jaw disease; luminescence; microbiology; nonlinear optical microscopy; pathogenicity; Raman spectrometry; suppuration; Bacteria; Face; Humans; Luminescent Measurements; Maxillary Diseases; Metal Nanoparticles; Nonlinear Optical Microscopy; Silver; Spectrum Analysis, Raman; Suppuration
Александров М. Т. Михаил Тимофеевич 0001-
Маргарян Э. Г. Эдита Гарниковна 1981-
Aleksandrov M. T. Mikhail Timofeevich 0001-
Margaryan E`. G. E`dita Garnikovna 1981-
Rationale for the application of surface-enhanced Raman scattering for identification of main pathogens of purulent-inflammatory diseases in maxillofacial area [Obosnovanie primeneniia éffekta gigantskogo ramanovskogo rasseianiia dlia identifikatsii osnovnykh vozbuditeleĭ gnoĭno-vospalitel'nykh protsessov cheliustno-litsevoĭ oblasti]
Rationale for the application of surface-enhanced Raman scattering for identification of main pathogens of purulent-inflammatory diseases in...
Текст визуальный непосредственный
Стоматология
Издательство Медиа Сфера
Т. 97, Вып. 1 С. 27-32
2018
Статья
antibiotic-resistance clinical microbiology microorganism indication raman peaks raman-spectrum library SERS-substrates suppurative surgical infection
colloidal silver metal nanoparticle silver bacterium classification devices face human isolation and purification jaw disease luminescence microbiology nonlinear optical microscopy pathogenicity Raman spectrometry suppuration Bacteria Face Humans Luminescent Measurements Maxillary Diseases Metal Nanoparticles Nonlinear Optical Microscopy Silver Spectrum Analysis, Raman Suppuration
The objective of the research was to elaborate experimental-theoretical and clinic-bacteriological rationale for the application of laser diagnostic for identification of main pathogens of purulent-inflammatory processes in maxillofacial area. For germs identification by giant Raman scattering effect SERS-substrate with nano silver metallic balls, reference strains (Ps. aeruginosa 27853 and S. aureus 25923) and clinical cultures of Staphylococcus, Bacillus and Escherichia coli were used. Using an example of purulent inflammation pathogens we considered that each of bacterial species is characterized by individual spectral lines of Raman scattering, which allows to identify them in short term (1-2 min). Moreover the proposed method is highly sensitive (105-106 CFU/ml). Creation of germs library and device portability makes use of laser diagnostic for express-indication purulent infections possible directly in clinical conditions. Thus, analytical capability, quick result, high sensitivi