Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
Moloudi R., Oh S., Yang C., Teo K. L., Lam A. T. -., Ibragimi V. M. M., Naing M. W.
Scientific Reports
Vol.8, Issue1, Num.12481
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1038/s41598-018-31019-y
Аннотация:
Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α) = 0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68[%]. Accordingly, the high-throughput trape
Ключевые слова:
article; cell suspension; filtration; flow rate; human; human cell; mesenchymal stem cell; bioreactor; cell culture technique; cell line; cytology; filtration; mesenchymal stem cell; procedures; suspension; Bioreactors; Cell Culture Techniques; Cell Line; Filtration; Humans; Mesenchymal Stem Cells; Suspensions
Язык текста: Английский
ISSN: 2045-2322
Moloudi R.
Oh S.
Yang C.
Teo K. L.
Lam A. T. -. A.T.-L.
Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-
Naing M. W.
Молоуди Р.
Ох С.
Янг C.
Тео К. Л.
Лам А. Т. -. А.Т.-Л.
Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-
Наинг М. W.
Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
Текст визуальный непосредственный
Scientific Reports
Vol.8, Issue1 Num.12481
2018
Статья
article cell suspension filtration flow rate human human cell mesenchymal stem cell bioreactor cell culture technique cell line cytology filtration mesenchymal stem cell procedures suspension Bioreactors Cell Culture Techniques Cell Line Filtration Humans Mesenchymal Stem Cells Suspensions
Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α) = 0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68[%]. Accordingly, the high-throughput trape