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2D/3D buccal epithelial cell self-assembling as a tool for cell phenotype maintenance and fabrication of multilayered epithelial linings in vitro

Zurina I. M., Shpichka A. I., Saburina I. N., Kosheleva N. V., Gorkun A. A., Ivukina E. A., Kuznetsova D. S., Zhang D., Rochev Yu. A., Butnaru D. V., Zharikova T. M., Istranova E. V., Zhang Y., Istranov L. P., Timashev P. S.
Biomedical Materials (Bristol): Materials for tissue engineering and regenerative medicine
Vol.13, Issue5, Num.054104
Опубликовано: 2018
Тип ресурса: Статья

DOI:10.1088/1748-605X/aace1c

Аннотация:
Maintaining the epithelial status of cells in vitro and fabrication of a multilayered epithelial lining is one of the key problems in the therapy using cell technologies. When cultured in a monolayer, epithelial cells change their phenotype from epithelial to epithelial-mesenchymal or mesenchymal that makes it difficult to obtain a sufficient number of cells in a 2D culture and to use them in tissue engineering. Here, using buccal epithelial cells from the oral mucosa, we developed a novel approach to recover and maintain the stable cell phenotype and form a multilayered epithelial lining in vitro via the 2D/3D cell self-assembling. Transitioning the cells from the monolayer to non-adhesive 3D culture conditions led to formation of self-assembling spheroids, with restoration of their epithelial characteristics after epithelial-mesenchymal transition. In 7 days, the cells within spheroids restored the apical-basal polarity, and the formation of both tight (ZO1) and adherent (E-cadherin)
Ключевые слова:
2D/3D self-assembling; 3D culture; buccal epithelial cells; buccal mucosa; cell spheroids; multilayered epithelial lining; tissue engineered urethra
Cells; Cytology; Fabrication; Linings; Monolayers; Restoration; Tissue; Tissue engineering; 3-d cultures; Buccal mucosa; Cell spheroids; Epithelial cells; Multi-layered; Self-assembling; Cell engineering; protein ZO1; uvomorulin; cadherin; CDH1 protein, human; collagen; leukocyte antigen; polyester; polylactide; protein ZO1; TJP1 protein, human; Article; cheek cell; controlled study; epithelium; epithelium cell; human; human cell; human tissue; in vitro study; mouth mucosa; tumor spheroid; biopsy; cell adhesion; cell culture technique; cell junction; cell proliferation; chemistry; cytology; epithelium; epithelium cell; metabolism; multicellular spheroid; phenotype; physiology; procedures; regenerative medicine; tissue engineering; transmission electron microscopy; urothelium; Antigens, CD; Biopsy; Cadherins; Cell Adhesion; Cell Culture Techniques; Cell Proliferation; Collagen; Epithelial Cells; Epithelium; Humans; Intercellular Junctions; Microscopy, Electron, Transmission; Mouth Mucos
Язык текста: Английский
ISSN: 1748-605X
Zurina I. M. Irina Mikhaylovna 1988-
Shpichka A. I. Anastasiya Iosifovna 1991-
Saburina I. N.
Kosheleva N. V.
Gorkun A. A. Anastasiya Alekseevna 1986-
Ivukina E. A. Ekaterina Andreevna 1986-
Kuznetsova D. S.
Zhang D.
Rochev Yu. A. Yurij Alekseevich 1961-
Butnaru D. V. Denis Viktorovich 1978-
Zharikova T. M. Tat`yana Mikhaylovna 1987-
Istranova E. V. Elena Viktorovna 1951-
Zhang Y.
Istranov L. P. Leonid Prokofyevich 1937-
Timashev P. S. Petr Sergeevich 1978-
Зурина И. М. Ирина Михайловна 1988-
Шпичка А. И. Анастасия Иосифовна 1991-
Сабурина И. Н.
Кошелева Н. В.
Горкун А. А. Анастасия Алексеевна 1986-
Ивукина Е. А. Екатерина Андреевна 1986-
Кузнецова Д. С.
Жанг Д.
Рочев Ю. А. Юрий Алексеевич 1961-
Бутнару Д. В. Денис Викторович 1978-
Жарикова Т. М. Татьяна Михайловна 1987-
Истранова Е. В. Елена Викторовна 1951-
Жанг Y.
Истранов Л. П. Леонид Прокофьевич 1937-
Тимашев П. С. Петр Сергеевич 1978-
2D/3D buccal epithelial cell self-assembling as a tool for cell phenotype maintenance and fabrication of multilayered epithelial linings in vitro
Текст визуальный непосредственный
Biomedical Materials (Bristol): Materials for tissue engineering and regenerative medicine
Institute of Physics and IOP Publishing Limited
Vol.13, Issue5 Num.054104
2018
Статья
2D/3D self-assembling 3D culture buccal epithelial cells buccal mucosa cell spheroids multilayered epithelial lining tissue engineered urethra
Cells Cytology Fabrication Linings Monolayers Restoration Tissue Tissue engineering 3-d cultures Buccal mucosa Cell spheroids Epithelial cells Multi-layered Self-assembling Cell engineering protein ZO1 uvomorulin cadherin CDH1 protein, human collagen leukocyte antigen polyester polylactide protein ZO1 TJP1 protein, human Article cheek cell controlled study epithelium epithelium cell human human cell human tissue in vitro study mouth mucosa tumor spheroid biopsy cell adhesion cell culture technique cell junction cell proliferation chemistry cytology epithelium epithelium cell metabolism multicellular spheroid phenotype physiology procedures regenerative medicine tissue engineering transmission electron microscopy urothelium Antigens, CD Biopsy Cadherins Cell Adhesion Cell Culture Techniques Cell Proliferation Collagen Epithelial Cells Epithelium Humans Intercellular Junctions Microscopy, Electron, Transmission Mouth Mucos
Maintaining the epithelial status of cells in vitro and fabrication of a multilayered epithelial lining is one of the key problems in the therapy using cell technologies. When cultured in a monolayer, epithelial cells change their phenotype from epithelial to epithelial-mesenchymal or mesenchymal that makes it difficult to obtain a sufficient number of cells in a 2D culture and to use them in tissue engineering. Here, using buccal epithelial cells from the oral mucosa, we developed a novel approach to recover and maintain the stable cell phenotype and form a multilayered epithelial lining in vitro via the 2D/3D cell self-assembling. Transitioning the cells from the monolayer to non-adhesive 3D culture conditions led to formation of self-assembling spheroids, with restoration of their epithelial characteristics after epithelial-mesenchymal transition. In 7 days, the cells within spheroids restored the apical-basal polarity, and the formation of both tight (ZO1) and adherent (E-cadherin)