Osteoinducing scaffolds with multi-layered biointerface

Ivukina E. A., Grinchenko V. D., Churbanov S. N., Minaev N. V., Shavkuta B. S., Melnikov P. A., Butnaru D. V., Rochev Yu. A., Bagratashvili V. N., Timashev P. S.

Biomedical Materials (Bristol): Materials for tissue engineering and regenerative medicine

Vol.13, Issue5, Num.054103

Опубликовано: 2018

Тип ресурса: Статья

DOI:10.1088/1748-605X/aac4cb

Аннотация:

This study was aimed to design and characterise hybrid tissue-engineered constructs composed of osteoinducing polylactide-based scaffolds with multi-layered cellular biointerface for bone tissue reconstruction. Three-dimensional scaffolds with improved hydrophilic and osteoinducing properties were produced using the surface-selective laser sintering (SSLS) method. The designed scaffold pattern had dimensions of 8 ×8 ×2.5 mm and ladder-like pores (∼700 μm in width). Hyaluronic acid-coated polylactide microparticles (∼100 μm in diameter) were used as building blocks and water was used as the photosensitizer for SSLS followed by photocross-linking with Irgacure 2959 photoinitiator. Resulting scaffolds provided successful adhesion and expansion of human bone marrow mesenchymal stromal cells from a single-cell suspension. Induced calcium deposition by the cells associated with osteogenic differentiation was detected in 7-21 days of culturing in basal medium. The values were up to 60[%] higher

Ключевые слова:

mesenchymal stromal cells; multi-layered cell sheets; osteoinduction; polylactide; surface-selective laser sintering; tissue-engineering

3D printers; Bone; Cell culture; Cell engineering; Cells; Hyaluronic acid; Laser heating; Photosensitizers; Polyesters; Sintering; Tissue; Tissue engineering; Cell sheet; Mesenchymal stromal cells; Osteoinduction; Poly lactide; Surface-selective laser sintering; Scaffolds (biology); calcium; hyaluronic acid; photoinitiator; polylactide; biomaterial; collagen; microsphere; polyester; animal cell; Article; BMSC cell line; bone development; bone tissue; cell differentiation; cell invasion; cell structure; cell suspension; cell viability; cellular distribution; contact angle; controlled study; cytotoxicity; flow cytometry; heating; human; human cell; human tissue; hydrophilicity; in vitro study; melting point; mouse; MTT assay; nonhuman; phenotype; polymerization; porosity; scanning electron microscopy; tissue engineering; tissue regeneration; 3T3 cell line; animal; bone marrow cell; bone prosthesis; chemistry; culture medium; cytology; drug effect; fibroblast; mesenchymal stem cell; osteo

Язык текста: Английский

ISSN: 1748-605X

Ivukina E. A. Ekaterina Andreevna 1986-

Grinchenko V. D. Varvara Dmitrievna 1995-

Churbanov S. N. Semen Nikolaevich 1992-

Minaev N. V.

Shavkuta B. S. Boris Sergeevich 1992-

Melnikov P. A.

Butnaru D. V. Denis Viktorovich 1978-

Rochev Yu. A. Yurij Alekseevich 1961-

Bagratashvili V. N.

Timashev P. S. Petr Sergeevich 1978-

Ивукина Е. А. Екатерина Андреевна 1986-

Гринченко В. Д. Варвара Дмитриевна 1995-

Чурбанов С. Н. Семен Николаевич 1992-

Минаев Н. В.

Шавкута Б. С. Борис Сергеевич 1992-

Мелников П. А.

Бутнару Д. В. Денис Викторович 1978-

Рочев Ю. А. Юрий Алексеевич 1961-

Баграташвили В. Н.

Тимашев П. С. Петр Сергеевич 1978-

Osteoinducing scaffolds with multi-layered biointerface

Текст визуальный непосредственный

Biomedical Materials (Bristol): Materials for tissue engineering and regenerative medicine

Institute of Physics and IOP Publishing Limited

Vol.13, Issue5 Num.054103

2018

Статья

mesenchymal stromal cells multi-layered cell sheets osteoinduction polylactide surface-selective laser sintering tissue-engineering

3D printers Bone Cell culture Cell engineering Cells Hyaluronic acid Laser heating Photosensitizers Polyesters Sintering Tissue Tissue engineering Cell sheet Mesenchymal stromal cells Osteoinduction Poly lactide Surface-selective laser sintering Scaffolds (biology) calcium hyaluronic acid photoinitiator polylactide biomaterial collagen microsphere polyester animal cell Article BMSC cell line bone development bone tissue cell differentiation cell invasion cell structure cell suspension cell viability cellular distribution contact angle controlled study cytotoxicity flow cytometry heating human human cell human tissue hydrophilicity in vitro study melting point mouse MTT assay nonhuman phenotype polymerization porosity scanning electron microscopy tissue engineering tissue regeneration 3T3 cell line animal bone marrow cell bone prosthesis chemistry culture medium cytology drug effect fibroblast mesenchymal stem cell osteo

This study was aimed to design and characterise hybrid tissue-engineered constructs composed of osteoinducing polylactide-based scaffolds with multi-layered cellular biointerface for bone tissue reconstruction. Three-dimensional scaffolds with improved hydrophilic and osteoinducing properties were produced using the surface-selective laser sintering (SSLS) method. The designed scaffold pattern had dimensions of 8 ×8 ×2.5 mm and ladder-like pores (∼700 μm in width). Hyaluronic acid-coated polylactide microparticles (∼100 μm in diameter) were used as building blocks and water was used as the photosensitizer for SSLS followed by photocross-linking with Irgacure 2959 photoinitiator. Resulting scaffolds provided successful adhesion and expansion of human bone marrow mesenchymal stromal cells from a single-cell suspension. Induced calcium deposition by the cells associated with osteogenic differentiation was detected in 7-21 days of culturing in basal medium. The values were up to 60[%] higher