Multilayered Cultures of NSCLC cells grown at the Air-Liquid Interface allow the efficacy testing of inhaled anti-cancer drugs
Movia D., Bazou D., Volkov Yu. T., Prina-Mello A.
Scientific Reports
Vol.8, Issue1, Num.12920
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1038/s41598-018-31332-6
Аннотация:
Evidence supports the advantages of inhalation over other drug-administration routes in the treatment of lung diseases, including cancer. Although data obtained from animal models and conventional in vitro cultures are informative, testing the efficacy of inhaled chemotherapeutic agents requires human-relevant preclinical tools. Such tools are currently unavailable. Here, we developed and characterized in vitro models for the efficacy testing of inhaled chemotherapeutic agents against non-small-cell lung cancer (NSCLC). These models recapitulated key elements of both the lung epithelium and the tumour tissue, namely the direct contact with the gas phase and the three-dimensional (3D) architecture. Our in vitro models were formed by growing, for the first time, human adenocarcinoma (A549) cells as multilayered mono-cultures at the Air-Liquid Interface (ALI). The in vitro models were tested for their response to four benchmarking chemotherapeutics, currently in use in clinics, demonstrat
Ключевые слова:
antineoplastic agent; A-549 cell line; cell culture technique; cell proliferation; cytology; drug effect; human; lung tumor; metabolism; multicellular spheroid; non small cell lung cancer; tumor cell line; A549 Cells; Antineoplastic Agents; Carcinoma, Non-Small-Cell Lung; Cell Culture Techniques; Cell Line, Tumor; Cell Proliferation; Humans; Lung Neoplasms; Spheroids, Cellular
Язык текста: Английский
ISSN: 2045-2322
Movia D.
Bazou D.
Volkov Yu. T. Yurij Tarasovich 1962-
Prina-Mello A.
Мовиа Д.
Базоу Д.
Волков Ю. Т. Юрий Тарасович 1962-
Прина-Мелло А.
Multilayered Cultures of NSCLC cells grown at the Air-Liquid Interface allow the efficacy testing of inhaled anti-cancer drugs
Текст визуальный непосредственный
Scientific Reports
Vol.8, Issue1 Num.12920
2018
Статья
antineoplastic agent A-549 cell line cell culture technique cell proliferation cytology drug effect human lung tumor metabolism multicellular spheroid non small cell lung cancer tumor cell line A549 Cells Antineoplastic Agents Carcinoma, Non-Small-Cell Lung Cell Culture Techniques Cell Line, Tumor Cell Proliferation Humans Lung Neoplasms Spheroids, Cellular
Evidence supports the advantages of inhalation over other drug-administration routes in the treatment of lung diseases, including cancer. Although data obtained from animal models and conventional in vitro cultures are informative, testing the efficacy of inhaled chemotherapeutic agents requires human-relevant preclinical tools. Such tools are currently unavailable. Here, we developed and characterized in vitro models for the efficacy testing of inhaled chemotherapeutic agents against non-small-cell lung cancer (NSCLC). These models recapitulated key elements of both the lung epithelium and the tumour tissue, namely the direct contact with the gas phase and the three-dimensional (3D) architecture. Our in vitro models were formed by growing, for the first time, human adenocarcinoma (A549) cells as multilayered mono-cultures at the Air-Liquid Interface (ALI). The in vitro models were tested for their response to four benchmarking chemotherapeutics, currently in use in clinics, demonstrat