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Genetic methods for detecting astrocytes, neurons and neurogenesis [Geneticke metode detekcije astrocita, neurona i neurogeneze]

Shusharina N. N., Silina E. V., Vasilyev A. A., Dominova I. N., Stupin V. A., Sinelnicova T. G., Sotnikov E. B., Turkin A. V., Patrushev M. V.
Serbian Journal of Experimental and Clinical Research
Vol.19, Issue2, P. 175-184
Опубликовано: 2018
Тип ресурса: Статья

DOI:10.2478/SJECR_2018_0013

Аннотация:
Two sets of reactants for modelling neurogenesis (SRMN) were developed based on the designed and tested genetic structures of lentiviral vectors. SRMN-1 contains the genetic construct LVV-GFAP-GCaMP3 and is intended for cellspecific transduction in astroglia cells. SRMN-2 contains the genetic construct LVV-PRSx8-TN-XXL and is intended for the phenotype-specific transduction in neurons. The present study examined SRMN-1 and SRMN-2 samples and assessed their efficiency in vitro and in vivo in Norvegicus rats. Specificity to particular cell types for all SRMN samples exceeded 97[%]. The number of induced signalling cascades was determined via activation of intracellular ingsignalling cascades in neurons and astrocytes (purinergic receptors and β-adrenoceptors). The results demonstrated dynamic recording of fluorescent signals and a two-fold increase in intensity after addition of the activator in all samples. The experimental SRMN samples revealed successful and stable transfection of catec
Ключевые слова:
Genetic structure; Neurogenesis astrocytes; Neurons; Signalling cascades; Viral vector
beta adrenergic receptor; purinergic receptor; animal cell; Article; astrocyte; catecholamine nerve cell; gene structure; genetic procedures; in vitro study; in vivo study; intracellular signaling; nervous system development; nonhuman; rat
Язык текста: Английский
ISSN: 1820-8665
Shusharina N. N.
Silina E. V. Ekaterina Vladimirovna 1980-
Vasilyev A. A.
Dominova I. N.
Stupin V. A.
Sinelnicova T. G.
Sotnikov E. B.
Turkin A. V.
Patrushev M. V.
Шушарина Н. Н.
Силина Е. В. Екатерина Владимировна 1980-
Васильев А. А.
Доминова И. Н.
Стюпин В. А.
Синелниcова Т. Г.
Сотников Е. Б.
Тюркин А. В.
Патрушев М. В.
Genetic methods for detecting astrocytes, neurons and neurogenesis [Geneticke metode detekcije astrocita, neurona i neurogeneze]
Текст визуальный непосредственный
Serbian Journal of Experimental and Clinical Research
Vol.19, Issue2 P. 175-184
2018
Статья
Genetic structure Neurogenesis astrocytes Neurons Signalling cascades Viral vector
beta adrenergic receptor purinergic receptor animal cell Article astrocyte catecholamine nerve cell gene structure genetic procedures in vitro study in vivo study intracellular signaling nervous system development nonhuman rat
Two sets of reactants for modelling neurogenesis (SRMN) were developed based on the designed and tested genetic structures of lentiviral vectors. SRMN-1 contains the genetic construct LVV-GFAP-GCaMP3 and is intended for cellspecific transduction in astroglia cells. SRMN-2 contains the genetic construct LVV-PRSx8-TN-XXL and is intended for the phenotype-specific transduction in neurons. The present study examined SRMN-1 and SRMN-2 samples and assessed their efficiency in vitro and in vivo in Norvegicus rats. Specificity to particular cell types for all SRMN samples exceeded 97[%]. The number of induced signalling cascades was determined via activation of intracellular ingsignalling cascades in neurons and astrocytes (purinergic receptors and β-adrenoceptors). The results demonstrated dynamic recording of fluorescent signals and a two-fold increase in intensity after addition of the activator in all samples. The experimental SRMN samples revealed successful and stable transfection of catec