Rational Surface Design of Upconversion Nanoparticles with Polyethylenimine Coating for Biomedical Applications: Better Safe than Brighter?

Guller A. E., Nadort A., Generalova A. N., Khaydukov E. V., Nechaev A. V., Kornienko I. A., Petersen E. V., Liang L., Shekhter A. B., Qian Y., Goldys E. M., Zvyagin A. V.

ACS Biomaterial Science and Engineering

Vol.4, Issue9, P. 3143-3153

Опубликовано: 2018

Тип ресурса: Статья

DOI:10.1021/acsbiomaterials.8b00633

Аннотация:

Upconversion nanoparticles (UCNPs) coated with polyethylenimine (PEI) are popular background-free optical contrast probes and efficient drug and gene delivery agents attracting attention in science, industry, and medicine. Their unique optical properties are especially useful for subsurface nanotheranostics applications, in particular, in skin. However, high cytotoxicity of PEI limits safe use of UCNP@PEI, and this represents a major barrier for clinical translation of UCNP@PEI-based technologies. Our study aims to address this problem by exploring additional surface modifications to UCNP@PEI to create less toxic and functional nanotheranostic materials. We designed and synthesized six types of layered polymer coatings that envelop the original UCNP@PEI surface, five of which reduced the cytotoxicity to human skin keratinocytes under acute (24 h) and subacute (120 h) exposure. In parallel, we examined the photoluminescence spectra and lifetime of the surface-modified UCNP@PEI. To quant

Ключевые слова:

cytotoxicity; photoluminescence; skin; surface modification; upconversion nanoparticles

Biocompatibility; Biological materials; Cytotoxicity; Drug delivery; Economic and social effects; Gene transfer; Luminance; Mass spectrometry; Medical applications; Nanoparticles; Photoluminescence; Biomedical applications; Clinical application; Clinical translation; Colloidal concentrations; Photoluminescence signals; Photoluminescence spectrum; Photophysical properties; Upconversion nanoparticles; Plastic coatings; polyethyleneimine; tetramethylammonium hydroxide; upconversion nanoparticle; Article; cell viability; comparative study; cytotoxicity; gene delivery system; HaCat cell line; human; human cell; image analysis; inductively coupled plasma mass spectrometry; keratinocyte; molecular weight; phase transition; photoluminescence; photon correlation spectroscopy; priority journal; room temperature; surface charge; surface property; survival rate; theranostic nanomedicine; transmission electron microscopy

Язык текста: Английский

ISSN: 2373-9878

Guller A. E. Anna Evgenyevna 1973-

Nadort A.

Generalova A. N.

Khaydukov E. V. Evgenij Valeryevich 1984-

Nechaev A. V.

Kornienko I. A. Inna Aleksandrovna 1991-

Petersen E. V. Elena Vladimirovna 1979-

Liang L.

Shekhter A. B. Anatolij Borukhovich 1933-

Qian Y.

Goldys E. M.

Zvyagin A. V. Andrej Vasilyevich 1961-

Гуллер А. Е. Анна Евгеньевна 1973-

Надорт А.

Генералова А. Н.

Хайдуков Е. В. Евгений Валерьевич 1984-

Нечаев А. В.

Корниенко И. А. Инна Александровна 1991-

Петерсен Е. В. Елена Владимировна 1979-

Лианг Л.