Comparison of spectrophotometric methods of total flavonoid assay based on complex formation with aluminum chloride as applied to multicomponent...
Struchkov P., Beloborodov V. L., Kolkhir V., Voskoboynikova I., Savvateev A. M.
Journal of Pharmaceutical Negative Results
Vol.9, Issue1, P. 1-7
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.4103/jpnr.JPNR_22_17
Аннотация:
Objective: The objective was to compare the complex formation of flavones (luteolin, apigenin), flavonols (morin, quercetin, and rutin), flavanones (naringenin), flavanonols (dihydroquercetin), caffeic, and ferulic acids with aluminum ion using two spectrophotometric methods and the application of these methods to estimate the flavonoid content in the angionorm herbal product. Materials and Methods: Method 1 included direct complex formation with aluminum chloride, and Method 2 involved preliminary nitrozation and subsequent complex formation of nitroso-derivatives with aluminum chloride. 2,4-dinitrophenylhydrazine method was also tested. Results: The conjugation system increase in the chromophore structure of nitroso derivatives is reflected by the hyperchromic effect of all substances (from 1.4 to 4-fold). The largest bathochromic shift of nitroso derivatives was seen for rutin, luteolin, and dihydroquercetin (to 510-530 nm). The total flavonoids estimation in the extract of the four
Ключевые слова:
Flavanones; flavanonols; flavones; flavonols; nitrosation
2,4 dinitrophenylhydrazine; alcohol; aluminum chloride; apigenin; caffeic acid; coumaric acid; ferulic acid; flavonoid; herbaceous agent; luteolin; morin; naringenin; nitroso derivative; quercetin; rutoside; taxifolin; Aesculus hippocastanum; Article; chemical reaction; chemical structure; chromatophore; complex formation; conjugation; Crataegus; Glycyrrhiza uralensis; intermethod comparison; nonhuman; protein analysis; protein content; protein determination; Rosa canina; ultraviolet spectrophotometry
Язык текста: Английский
ISSN: 2229-7723
Struchkov P.
Beloborodov V. L. Vladimir Leonidovich 1948-
Kolkhir V.
Voskoboynikova I.
Savvateev A. M. Aleksej Mikhaylovich 1974-
Стручков П.
Белобородов В. Л. Владимир Леонидович 1948-
Колхир В.
Воскобоyникова И.
Савватеев А. М. Алексей Михайлович 1974-
Comparison of spectrophotometric methods of total flavonoid assay based on complex formation with aluminum chloride as applied to multicomponent herbal drug angionorm
Comparison of spectrophotometric methods of total flavonoid assay based on complex formation with aluminum chloride as applied to multicomponent...
Текст визуальный непосредственный
Journal of Pharmaceutical Negative Results
Vol.9, Issue1 P. 1-7
2018
Статья
Flavanones flavanonols flavones flavonols nitrosation
2,4 dinitrophenylhydrazine alcohol aluminum chloride apigenin caffeic acid coumaric acid ferulic acid flavonoid herbaceous agent luteolin morin naringenin nitroso derivative quercetin rutoside taxifolin Aesculus hippocastanum Article chemical reaction chemical structure chromatophore complex formation conjugation Crataegus Glycyrrhiza uralensis intermethod comparison nonhuman protein analysis protein content protein determination Rosa canina ultraviolet spectrophotometry
Objective: The objective was to compare the complex formation of flavones (luteolin, apigenin), flavonols (morin, quercetin, and rutin), flavanones (naringenin), flavanonols (dihydroquercetin), caffeic, and ferulic acids with aluminum ion using two spectrophotometric methods and the application of these methods to estimate the flavonoid content in the angionorm herbal product. Materials and Methods: Method 1 included direct complex formation with aluminum chloride, and Method 2 involved preliminary nitrozation and subsequent complex formation of nitroso-derivatives with aluminum chloride. 2,4-dinitrophenylhydrazine method was also tested. Results: The conjugation system increase in the chromophore structure of nitroso derivatives is reflected by the hyperchromic effect of all substances (from 1.4 to 4-fold). The largest bathochromic shift of nitroso derivatives was seen for rutin, luteolin, and dihydroquercetin (to 510-530 nm). The total flavonoids estimation in the extract of the four