Аннотация:
The objective of the present study was the development and validation of the rapid reproducible method for the identification of ethyl glucuronide and ethyl sulfate allowing to store and transport the study specimens without the loss of the substances of interest by placing the samples on the paper. We have developed the validated technique for the detection and quantitative determination of ethyl glucuronide and ethyl sulfate in the cadaveric blood and urine by means of low-resolution tandem mass-spectroscopy with the use of deuterated derivatives of these substances as the internal standards. The low threshold for quantitative determination of both above substances is 50 ng/ml for the blood and 100 ng/ml for the urine. The method is characterized by the accuracy and precision with the coefficient of variation below 15[%] and the influence of the matrix with the coefficient of variation below 15[%]. The evaluation of stability of the two analytes in blood when stored in the dry condition
Ключевые слова:
Ethyl alcohol; Ethyl glucuronide; Ethyl sulfate; High performance liquid chromatography; Mass-spectroscopy
diethyl sulfate; ethyl glucuronide; glucuronic acid; sulfate; autopsy; blood; diagnosis; drinking behavior; high performance liquid chromatography; human; procedures; reproducibility; tandem mass spectrometry; Alcohol Drinking; Autopsy; Chromatography, High Pressure Liquid; Diagnosis; Glucuronates; Humans; Reproducibility of Results; Sulfuric Acid Esters; Tandem Mass Spectrometry
Ерошченко Н. Н.
Барсегян С. С.
Кирюшин А. Н. Алексей Николаевич 1987-
Туаева Н. О. Наталья Олеговна 1976-
Носырев А. Е. Александр Евгеньевич 1958-
Саломатин В. Е.
Eroshchenko N. N.
Barsegyan S. S.
Kiryushin A. N. Aleksej Nikolaevich 1987-
Tuaeva N. O. Natal`ya Olegovna 1976-
Nosy'rev A. E. Aleksandr Evgenyevich 1958-
Salomatin V. E.
The development and validation of the method for the identification of ethyl glucuronide and ethyl sulfate as the markers of the consumption of ethyl alcohol during one’s lifetime
The development and validation of the method for the identification of ethyl glucuronide and ethyl sulfate as the markers of the consumption of...
Текст визуальный непосредственный
Судебно-медицинская экспертиза
Издательство Медиа Сфера
Т. 61, Вып. 4 С. 42-47
2018
Статья
Ethyl alcohol Ethyl glucuronide Ethyl sulfate High performance liquid chromatography Mass-spectroscopy
diethyl sulfate ethyl glucuronide glucuronic acid sulfate autopsy blood diagnosis drinking behavior high performance liquid chromatography human procedures reproducibility tandem mass spectrometry Alcohol Drinking Autopsy Chromatography, High Pressure Liquid Diagnosis Glucuronates Humans Reproducibility of Results Sulfuric Acid Esters Tandem Mass Spectrometry
The objective of the present study was the development and validation of the rapid reproducible method for the identification of ethyl glucuronide and ethyl sulfate allowing to store and transport the study specimens without the loss of the substances of interest by placing the samples on the paper. We have developed the validated technique for the detection and quantitative determination of ethyl glucuronide and ethyl sulfate in the cadaveric blood and urine by means of low-resolution tandem mass-spectroscopy with the use of deuterated derivatives of these substances as the internal standards. The low threshold for quantitative determination of both above substances is 50 ng/ml for the blood and 100 ng/ml for the urine. The method is characterized by the accuracy and precision with the coefficient of variation below 15[%] and the influence of the matrix with the coefficient of variation below 15[%]. The evaluation of stability of the two analytes in blood when stored in the dry condition