A new method for the determination of ammonium in the vitreous humour based on capillary electrophoresis and its preliminary application in...
Gottardo R., Palacio C., Shestakova K. M., Moskaleva N. E., Bortolotti F., Tal`yaro F.
Clinical Chemistry and Laboratory Medicine
Vol.57, Issue4, P. 504-509
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1515/cclm-2018-0384
Аннотация:
Background: Although the post-mortem increase of ammonium in biological fluids is well known, ammonium analysis in vitreous humour has never been used in recent times for the determination of the post-mortem interval. The present work represents a new application of capillary electrophoresis with indirect UV detection in the field of forensic analysis. Methods: The electrophoretic separation was carried out in a running buffer made of 5 mM imidazole, 5 mM 18-crown-6 ether and 6 mM d,l-α-hydroxybutyric acid (HIBA). To overcome the lack of optical absorption of ammonium, indirect UV detection was applied. The used wavelength was 214 nm. Results: The method showed good linearity in the concentration range from 0.16 to 5.0 mM. The limit of detection, 0.039 mmol/L, was established on the basis of the linearity curve. Precision and bias studies carried out on the pure ammonium solutions and in real biological samples, revealed [%]RSDs well below 20[%]. A preliminary application to real cases whe
Ключевые слова:
Ammonium; Capillary electrophoresis; Post mortem interval; Vitreous humour
ammonia; ether; imidazole; ammonium derivative; absorption; adult; aged; Article; calibration; capillary electrophoresis; concentration (parameter); female; human; limit of detection; limit of quantitation; male; measurement precision; middle aged; preliminary data; priority journal; protein determination; reproducibility; time of death; very elderly; vitreous body; young adult; capillary electrophoresis; chemistry; forensic medicine; procedures; ultraviolet spectrophotometry; vitreous body; Adult; Aged; Aged, 80 and over; Ammonium Compounds; Electrophoresis, Capillary; Female; Forensic Medicine; Humans; Male; Middle Aged; Spectrophotometry, Ultraviolet; Vitreous Body; Young Adult
Язык текста: Английский
ISSN: 1437-4331
Gottardo R.
Palacio C.
Shestakova K. M. Kseniya Mikhaylovna 1992-
Moskaleva N. E. Natal`ya Evgenyevna 1971-
Bortolotti F.
Tal`yaro F. Franko 1952-
Готтардо Р.
Палаcио C.
Шестакова К. М. Ксения Михайловна 1992-
Москалева Н. Е. Наталья Евгеньевна 1971-
Бортолотти Ф.
Тальяро Ф. Франко 1952-
A new method for the determination of ammonium in the vitreous humour based on capillary electrophoresis and its preliminary application in thanatochemistry
A new method for the determination of ammonium in the vitreous humour based on capillary electrophoresis and its preliminary application in...
Текст визуальный непосредственный
Clinical Chemistry and Laboratory Medicine
Walter de Gruyter GmbH
Vol.57, Issue4 P. 504-509
2019
Статья
Ammonium Capillary electrophoresis Post mortem interval Vitreous humour
ammonia ether imidazole ammonium derivative absorption adult aged Article calibration capillary electrophoresis concentration (parameter) female human limit of detection limit of quantitation male measurement precision middle aged preliminary data priority journal protein determination reproducibility time of death very elderly vitreous body young adult capillary electrophoresis chemistry forensic medicine procedures ultraviolet spectrophotometry vitreous body Adult Aged Aged, 80 and over Ammonium Compounds Electrophoresis, Capillary Female Forensic Medicine Humans Male Middle Aged Spectrophotometry, Ultraviolet Vitreous Body Young Adult
Background: Although the post-mortem increase of ammonium in biological fluids is well known, ammonium analysis in vitreous humour has never been used in recent times for the determination of the post-mortem interval. The present work represents a new application of capillary electrophoresis with indirect UV detection in the field of forensic analysis. Methods: The electrophoretic separation was carried out in a running buffer made of 5 mM imidazole, 5 mM 18-crown-6 ether and 6 mM d,l-α-hydroxybutyric acid (HIBA). To overcome the lack of optical absorption of ammonium, indirect UV detection was applied. The used wavelength was 214 nm. Results: The method showed good linearity in the concentration range from 0.16 to 5.0 mM. The limit of detection, 0.039 mmol/L, was established on the basis of the linearity curve. Precision and bias studies carried out on the pure ammonium solutions and in real biological samples, revealed [%]RSDs well below 20[%]. A preliminary application to real cases whe