Large-scale production of stem cells utilizing microcarriers: A biomaterials engineering perspective from academic research to commercialized products
Tavassoli H., Alhosseini S. N., Tay A., Chan P. P. Y., Weng O. S. K., Ibragimi V. M. M.
Biomaterials
Vol.181, P. 333-346
Опубликовано: 2018
Тип ресурса: Обзор
DOI:10.1016/j.biomaterials.2018.07.016
Аннотация:
Human stem cells, including pluripotent, embryonic and mesenchymal, stem cells play pivotal roles in cell-based therapies. Over the past decades, various methods for expansion and differentiation of stem cells have been developed to satisfy the burgeoning clinical demands. One of the most widely endorsed technologies for producing large cell quantities is using microcarriers (MCs) in bioreactor culture systems. In this review, we focus on microcarriers properties that can manipulate the expansion and fate of stem cells. Here, we provide an overview of commercially available MCs and focus on novel stimulus responsive MCs controlled by temperature, pH and field changes. Different features of MCs including composition, surface coating, morphology, geometry/size, surface functionalization, charge and mechanical properties, and their cellular effects are also highlighted. We then conclude with current challenges and outlook on this promising technology. © 2018 Elsevier Ltd
Ключевые слова:
Biomaterials; Microcarries; Microtechnology; Polymers; Stem cell; Tissue engineering
Biomaterials; Biomechanics; Cytology; Polymers; Stem cells; Tissue engineering; Bioreactor cultures; Cell based therapies; Commercialized products; Engineering perspective; Large scale productions; Microcarries; Microtechnology; Surface Functionalization; Cell engineering; biomaterial; biomaterial; polymer; cell differentiation; cell structure; cell therapy; controlled study; embryonic stem cell; human; human cell; mesenchymal stem cell; microcarrier culture; monolayer culture; pH; pluripotent stem cell; priority journal; regenerative medicine; Review; stem cell; temperature; cell culture; cell culture technique; chemistry; cytology; physiology; procedures; tissue engineering; Biocompatible Materials; Cell Culture Techniques; Cell Differentiation; Cells, Cultured; Humans; Hydrogen-Ion Concentration; Polymers; Stem Cells; Temperature; Tissue Engineering
Язык текста: Английский
ISSN: 1878-5905
Tavassoli H.
Alhosseini S. N.
Tay A.
Chan P. P. Y.
Weng O. S. K. Oh S.K.
Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-
Тавассоли Х.
Алхоссеини С. Н.
Тай А.
Чан П. П. Y.
Wенг О. С. К. Ох С.К.
Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-
Large-scale production of stem cells utilizing microcarriers: A biomaterials engineering perspective from academic research to commercialized products
Текст визуальный непосредственный
Biomaterials
Elsevier Science Publisher B.V.
Vol.181 P. 333-346
2018
Обзор
Biomaterials Microcarries Microtechnology Polymers Stem cell Tissue engineering
Biomaterials Biomechanics Cytology Polymers Stem cells Tissue engineering Bioreactor cultures Cell based therapies Commercialized products Engineering perspective Large scale productions Microcarries Microtechnology Surface Functionalization Cell engineering biomaterial biomaterial polymer cell differentiation cell structure cell therapy controlled study embryonic stem cell human human cell mesenchymal stem cell microcarrier culture monolayer culture pH pluripotent stem cell priority journal regenerative medicine Review stem cell temperature cell culture cell culture technique chemistry cytology physiology procedures tissue engineering Biocompatible Materials Cell Culture Techniques Cell Differentiation Cells, Cultured Humans Hydrogen-Ion Concentration Polymers Stem Cells Temperature Tissue Engineering
Human stem cells, including pluripotent, embryonic and mesenchymal, stem cells play pivotal roles in cell-based therapies. Over the past decades, various methods for expansion and differentiation of stem cells have been developed to satisfy the burgeoning clinical demands. One of the most widely endorsed technologies for producing large cell quantities is using microcarriers (MCs) in bioreactor culture systems. In this review, we focus on microcarriers properties that can manipulate the expansion and fate of stem cells. Here, we provide an overview of commercially available MCs and focus on novel stimulus responsive MCs controlled by temperature, pH and field changes. Different features of MCs including composition, surface coating, morphology, geometry/size, surface functionalization, charge and mechanical properties, and their cellular effects are also highlighted. We then conclude with current challenges and outlook on this promising technology. © 2018 Elsevier Ltd