Аннотация:
Разработка и валидация методики определения топирамата в плазме крови кроликов методом ВЭЖХ с предколоночной дериватизацией.
Тhe paper of Shohin I.E., Ramenskaya G.V., Medvedev Yu.V., Yarushok T.A., Sheveleva M.A.1 is devoted to development and validation of HPLC method determination of topiramate in rabbit plasma with precolumn derivatization. Topiramate was extracted from plasma by dichloromethane. Derivatization was performed by dansyl chloride in borate buffer. HPLC analysis was performed on C18 column (3 μm, 4,6 x 75 mm). Acetonitrile – 25 mM phosphate buffer pH 2.50 (30:70) at 1.0 mL/min was used as mobile phase. The standard curve was linear over the range 0.95 μg/ml to 47.32 μg/ml of topiramate in plasma. Within-run precision was 1.55 [%] to 2.12 [%] and between-run precision was 1.67 [%] to 8.09 [%] determined on spiked samples. The accuracy of the method was 1.14 [%] to 19.62 [%] (within-run) and 1.74 [%] to 19.25 (between-run). The lower limit of quantification was 0.95 μg/ ml. The method was applied to preclinical pharmacokinetics study of topiramate drug products in rabbits.
Раменская Г.В. Первый МГМУ им. И.М. Сеченова
Шевелева М.А. Первый МГМУ им. И.М. Сеченова
Шохин И.Е. Первый МГМУ им. И.М. Сеченова; «Технология лекарств»
Медведев Ю.В. Первый МГМУ им. И.М. Сеченова; «Технология лекарств»
Ярушок Т.А. Первый МГМУ им. И.М. Сеченова; «Технология лекарств»
Первый МГМУ им. И.М. Сеченова
First Moscow State Medical University
«Технология лекарств»
Drug Technology LLC
Ramenskaya G.V. First Moscow State Medical University
Sheveleva M.A. First Moscow State Medical University
Shokhin I.E. First Moscow State Medical University; Drug Technology LLC
Medvedev Y.V. Yu. V. First Moscow State Medical University; Drug Technology LLC
Yarushok T.A. First Moscow State Medical University; Drug Technology LLC
The determination of topiramate in plasma rabbit blood by HPLC with specific derivatization eng
Определение топирамата в плазме крови кроликов методом ВЭЖХ с предколоночной дериватизацией
Текст визуальный электронный
Сеченовский вестник
№ 3-4 P. 53-59
2011
топирамат
topiramate
плазма
plasma
кролики
rabbits
ВЭЖХ
HPLC
дериватизация
derivatization
Статья
СОЦИОЛОГИЯ МЕДИЦИНЫ
615.076.9: 616.85
Разработка и валидация методики определения топирамата в плазме крови кроликов методом ВЭЖХ с предколоночной дериватизацией.
Тhe paper of Shohin I.E., Ramenskaya G.V., Medvedev Yu.V., Yarushok T.A., Sheveleva M.A.1 is devoted to development and validation of HPLC method determination of topiramate in rabbit plasma with precolumn derivatization. Topiramate was extracted from plasma by dichloromethane. Derivatization was performed by dansyl chloride in borate buffer. HPLC analysis was performed on C18 column (3 μm, 4,6 x 75 mm). Acetonitrile – 25 mM phosphate buffer pH 2.50 (30:70) at 1.0 mL/min was used as mobile phase. The standard curve was linear over the range 0.95 μg/ml to 47.32 μg/ml of topiramate in plasma. Within-run precision was 1.55 [%] to 2.12 [%] and between-run precision was 1.67 [%] to 8.09 [%] determined on spiked samples. The accuracy of the method was 1.14 [%] to 19.62 [%] (within-run) and 1.74 [%] to 19.25 (between-run). The lower limit of quantification was 0.95 μg/ ml. The method was applied to preclinical pharmacokinetics study of topiramate drug products in rabbits.