Glybochko P.V.
Alekseenko S.N.
Gubareva E.A.
Kuevda E.V.
Basov A.A.
Sotnichenko A.S.
Dzhimak S.S.
Gumenyuk I.S.
Egiev I.K. I Kh
Chechelyan V.N.
Nakokhov R.Z.
Lyasota O.M.
Teterin Y.V. Yu V
I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Kuban State Medical University of Minzdrav of Russia, Krasnodar, Russia.
Kuban State University, Krasnodar, Russia.
Experimental development and rationale for a renal decellularization protocol with subsequent comprehensive assessment of the biological scaffold
Текст визуальный электронный
Urologiia (Moscow, Russia : 1999)
№ 2 С. 5-13
2017
EPR spectroscopy
decellularization
regenerative medicine
scaffold
tissue engineering
tissue-engineered kidney recellularization
Статья
Animals B01.050
Животные B01.050
Cell Separation E01.370.225.500.363 E05.200.500.363 E05.242.363
Клеток отделение E01.370.225.500.363 E05.200.500.363 E05.242.363
Kidney cytology A05.810.453
Kidney anatomy & histology A05.810.453
Почки цитология A05.810.453
Почки анатомия и гистология A05.810.453
Kidney Failure, Chronic therapy C12.050.351.968.419.780.750.500 C12.200.777.419.780.750.500 C12.950.419.780.750.500 C23.550.291.500.906.500
Почечная недостаточность функциональная, хроническая терапия C12.050.351.968.419.780.750.500 C12.200.777.419.780.750.500 C12.950.419.780.750.500 C23.550.291.500.906.500
Kidneys, Artificial E07.858.082.585
Почки искусственные E07.858.082.585
Rats B01.050.150.900.649.313.992.635.505.700
Крысы B01.050.150.900.649.313.992.635.505.700
Tissue Engineering E05.481.500.311.500 J01.293.069.249.500
Тканевая инженерия E05.481.500.311.500 J01.293.069.249.500
Tissue Scaffolds E07.206.627 E07.695.825
Ткани поддерживающие структуры E07.206.627 E07.695.825
Journal Article V02.600
Журнальная статья V02.600
Chronic renal failure (CRF) is one of the most challenging problems of contemporary medicine. Patients with chronic renal failure usually need renal replacement therapy as either hemodialysis, peritoneal dialysis or a kidney transplant. The latter is the most promising option for end-stage kidney disease. However, the shortage of donor organs, the complexity of their delivery, the difficulty in finding an immunologically compatible donor and the need for lifelong immunosuppression triggered advances in modern tissue engineering. In this field, the primary priority is focused on developing bioengineered scaffolds with subsequent recellularization with autologous cells. Using such constructs would allow for solving both ethical and immunological problems of transplantation. The aim of this pilot study was to develop a new method of renal decellularization using small laboratory animals.